FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1788_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1788_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228071
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC1950.052.05093145
TCGCCAT2000.050.749657145
GCGAAAG4500.046.7295152
AGCGAAA5350.037.9498521
CGAAAGC5750.036.5709273
TACATCG950.00472410230.531085
CCACGAC1804.701358E-628.194254145
ACCGTTC1902.1936651E-422.894588
GTGTAGG6100.022.5854931
ATGCCGG4109.404175E-1021.219368145
TCCGAAC1750.003655890520.717523
CACGAGC3508.74079E-820.714146145
TATACTG5650.020.534185
TCTCCGC4803.1468517E-1019.635284145
GAGCGAA2256.8616855E-419.3363511
CCGTTCA1900.00588886619.0788179
GTCCTAT10300.019.0078221
CCCACGC4654.50018E-918.709553145
TATAGGT6351.8189894E-1218.270574
GTGATCG2000.007917386518.1248769
Produced by FastQC (version 0.11.5)