FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1788_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1788_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475383
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC19240.13040681639953827No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG450.00963139148.3443155
GTGATCG1901.2914825E-1038.1588029
CGTAGAC1001.3899106E-436.2582363
CGAAAGC7650.036.0212553
GCGAAAG8050.033.3294262
AGCGAAA8500.031.5649241
TCGCCAT4350.029.999699145
ATGCCGG3151.8189894E-1229.920334145
TAACGAC1804.6936493E-628.200853
AGACGGC3503.6379788E-1226.928299145
CTACGCC1100.00967192126.3642679
GTAGTAC2051.2885343E-524.7617243
TCTCGTT3652.2009772E-1023.835379145
GAGCGAA2151.8637262E-523.6092151
GTATAAG6300.021.8693071
ACCTGCG6800.021.3240387
TACACCG2404.3570515E-521.1506395
TAACACG2659.337214E-519.1552944
GTGTAGA9100.019.1245771
GTATAGG3451.7399343E-618.91671
Produced by FastQC (version 0.11.5)