FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1787_d8eye_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1787_d8eye_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4736
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACACATGACACTTAAACCAATTTTCTATCATTGGATTTTTCAAT50.10557432432432433No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGTC100.006869444145.07
CCAGCAA100.006869444145.04
CTCCAGC100.006869444145.02
TATTGAA100.006869444145.02
CCGACAA100.006869444145.0145
GCTTATC100.006869444145.01
AAGTCCC100.006869444145.09
GGGGGGG5008.185452E-117.25140-144
Produced by FastQC (version 0.11.5)