FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1787_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1787_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139181
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC8800.035.4257145
GCGAAAG11900.032.2972342
AGCGAAA12900.028.668831
CGAAAGC14600.026.3244573
TCGCGTT3357.4578566E-1125.96983145
TCTCCGC15250.025.196411145
CGTAGAC1751.2546308E-424.8627763
ATACCGT2051.2888251E-524.7624976
GTATTAT33700.023.024141
CTCCGAC2852.7830538E-722.894455145
GTTACGT4006.89397E-1021.7545831
TCTCGTT11850.021.413239145
GTATTAG14750.020.6484171
CCGACTA4601.7826096E-1020.4937744
GTATATA19500.020.0811561
GCACCGT14350.019.7089256
TTACGTG4452.6029738E-919.5549932
TAGTACC11950.018.80799
TATTATC21450.018.2558862
ATGCCGG8850.018.022377145
Produced by FastQC (version 0.11.5)