FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1787_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1787_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108537
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGCG451.1928292E-464.459015
CACGAGC7000.039.356705145
GTTACGT3500.037.293261
TTACGTG4050.035.8105622
AGCGAAA8150.034.700271
GCGAAAG9250.031.3584382
CCGACTA4450.030.9620534
CTCCGAC1753.7775553E-628.999676145
CGAAAGC10350.028.025663
TATACTG11200.027.8411145
GTTGACA10900.026.6108881
GGTGCGA1751.256264E-424.8574548
GCTACTA3501.2914825E-1024.8568659
GTGCGAT1801.5211805E-424.1663979
TCTCCGC8300.023.584074145
TAGTACC10900.023.2850784
AGTCCGC2653.7835744E-621.886549145
ACGTCCG3655.693437E-921.849079
CAGTGCA14050.021.6774982
CCACCGA7050.021.6001151
Produced by FastQC (version 0.11.5)