FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1787_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1787_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176232
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1102.5465852E-1159.3397031
ACCGTAC701.6925325E-551.8045041
CTCCGAC604.9459253E-448.33237145
ATGCGCC1002.7349306E-643.49913145
CCGTACA1053.814057E-641.4453622
GTCGGCT1105.25046E-639.5614856
AGTCGAA750.001480540738.6823432
AAGTCGA800.002033137536.263151
GATAGCG1604.591675E-836.24928145
TATACTG6200.035.0948645
GATATAC1551.4606667E-632.7552033
GAACCGA950.00471826830.538696
TTACGTG2151.8038918E-830.361142
ACGTGTT2451.9645086E-929.603834
GTCGAAG1050.007693763427.6302413
ACATGGT6400.027.1985195
CTACTGA12300.027.1248116
TAGCATG10150.026.4392832
GTTATGT7150.026.37321
GACTTAA3657.2759576E-1225.8312871
Produced by FastQC (version 0.11.5)