FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1787_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1787_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249892
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG47850.3828330767778336No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG33150.26522291525987846No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT30250.24202091060667644No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC27330.21865889212827988No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA24240.19393675613573014No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT20890.16713444041565192No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA20390.1631340947857895No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG19380.1550533966134674No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18130.14505253253881137No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG17590.14073215925855992No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC17150.13721185510428102No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT17030.13625177215311404No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA16910.13529168920194704No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16750.1340115786003911No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG14500.11601002326601018No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC12730.10184879973629722No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG12510.10008864765915775No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC300.001935266972.505561
GGTACTG8150.067.612555
GCAGGTA8150.066.722912
GTACTGA8350.065.124756
CAGGTAC9150.060.2231983
AGCAGGT9950.054.6524351
ACTGATC11650.052.2745068
TCTCCGC3650.051.64367145
AGGTACT11650.049.7892234
CTGATCC12050.047.530969
GGATAGC18200.047.4074825
GTTACGT1109.9560566E-846.1399041
TTACGTG1109.9560566E-846.1399042
GTTATAG951.920258E-645.792991
GACGCGG803.7352667E-545.312347145
GACAGTA10400.044.615247
TTGGACA10800.043.6376084
ACGTCCG1002.734756E-643.499869
ATAGCAA20000.043.4998557
GTTGACA7050.042.166361
Produced by FastQC (version 0.11.5)