FastQCFastQC Report
Wed 2 May 2018
HNFV7BGX5_n04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFV7BGX5_n04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573621627
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29230590.5095796361945747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA968850.014.68196594-95
TGGTCGC2624950.012.80518794-95
TCGCCGT1408250.012.71811794-95
GTGGTCG3374500.011.59884194-95
GTCGCCG1965000.011.37728994-95
TCGGTGG5794200.010.9387194-95
GGTCGCC2566650.010.89902694-95
GCCGTAT1044300.010.87693894-95
GTAGATA1942700.010.60883494-95
GATCTCG9187900.010.59473694-95
ATCTCGG8377400.010.530230594-95
CTCGGTG6792350.010.44622194-95
TCTCGGT7499500.010.40673594-95
CGGTGGT5619400.09.65343794-95
TAGATCT12542250.09.31446994-95
CCGTATC946750.08.60669194-95
AGATCTC13053550.08.30992694-95
GTGTAGA18406700.08.3091794-95
TAGTGTA8190950.08.29379994-95
CGTATCA871000.08.089293594-95
Produced by FastQC (version 0.11.2)