FastQCFastQC Report
Wed 2 May 2018
HNFV7BGX5_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFV7BGX5_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573621627
Sequences flagged as poor quality0
Sequence length8
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCTAT11722403720.43577708411611No Hit
AGGCTATA11003321619.18219446771312No Hit
TAAGATTA10782571618.797358907808402No Hit
CTTCGCCT9837729217.150206228190207No Hit
GATCTGCT9542908016.636241645749525No Hit
AGATCTCG236899724.12989519309041Illumina Single End PCR Primer 1 (100% over 8bp)
GGGGGGGG19675420.3430034551329774No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)