FastQCFastQC Report
Wed 2 May 2018
HNFV7BGX5_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFV7BGX5_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573621627
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4212250.014.54649774-75
GCTTGAA5184600.014.17920560-61
CCGTCTT5459300.012.85741852-53
CGTCTTC5777700.012.61870852-53
GCCGTCT5848300.012.45094250-51
CTTGAAA6276000.011.449462-63
TGCTTGA6265850.011.34427160-61
ATGCCGT6937200.010.66907748-49
TATGCCG7378400.010.57932994-95
CGTATGC9424400.010.3771994-95
TCGTATG10488750.010.30220894-95
CTGCTTG7425550.010.02479458-59
AAAGGGG6049100.010.01870672-73
TGCCGTC7181250.09.98838650-51
CTCGTAT11468300.09.84289794-95
GTATGCC9262300.09.53433594-95
TTGAAAA8508050.09.15250962-63
AAGGGGG5573950.08.97987974-75
TCTCGTA12937900.08.8941294-95
ATCTCGT14871750.08.75413994-95
Produced by FastQC (version 0.11.2)