FastQCFastQC Report
Tue 7 Jan 2025
HNFNGDRX5_n02_Rep_3-2_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_3-2_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493441326
Sequences flagged as poor quality0
Sequence length64
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA343943656.970304915239304No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG298883866.057130691157392No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA289970955.876503136666749No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC251101615.088783544651872No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC225172924.563317017350914No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG214852744.354169962651244No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC209538994.246482387249421No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT187543903.8007335445592574No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT180080233.6494760473304986No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC174790013.542265327002627No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC155567493.152704927677662No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA135338022.7427378468093693No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC132483772.6848940901232905No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT115952232.349868644767706No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC103637792.10030624796108No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA98310841.992351163550497No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC94880761.922837731673897No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC84588611.7142587283011639No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC82322721.6683385777055895No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT79057761.6021714403385825No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG11723790.2375923819562693No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG11553160.23413442270135273No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA10139350.20548238393798415No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC10120200.20509429321694064No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG10028590.20323774016447094No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA9948020.20160492191932866No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG9845860.19953456431819008No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC8733370.17698902665481245No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA8633850.17497217085542607No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC8606580.17441952156232654No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA7964760.16141250398634022No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA7923490.16057613301728196No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT7684180.15572631628344807No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA7531410.1526303048237188No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC7315730.14825936974723514No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT7080910.14350054660804798No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG7047280.14281900661072722No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA6996960.14179922984399568No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA6495620.13163915662791487No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA6284940.12736955072141648No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC6238770.1264338771657727No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC6231450.1262855312609143No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA6151810.12467156024138927No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA6025660.12211502528266148No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC5931150.12019970131160031No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA5907470.11971980636255018No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC5767830.11688988530320218No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGG5330150.10801993507937355No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG5024870.10183318127675427No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGG4978480.10089304923763115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)