FastQCFastQC Report
Tue 24 Dec 2024
HNFNGDRX5_n02_Rep_3-2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_3-2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5217343
Sequences flagged as poor quality0
Sequence length6
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGA342253065.59909900499163No Hit
TCTTGA2618325.018493129548891No Hit
TCGTGT2217484.250209349854897No Hit
TAAACA1390532.665207175376432No Hit
TCAGGA701321.3442091118026935No Hit
TCAACA629931.207377011632166No Hit
TATGGA606641.1627374316773882No Hit
TCGTGA542881.0405296335701908No Hit
TCTGTA514090.985348289349579No Hit
TCGGGT505900.9696506440155458No Hit
TCGGGA480740.9214268642103846No Hit
TCTGCA431370.8268001547914331No Hit
TCTTCA424960.8145142077107063No Hit
TCACCA368750.7067773769138813No Hit
TCAGCA297650.5705011152228251No Hit
TCATCA269860.5172364554141831No Hit
TCTTGT261110.5004654668094468No Hit
TCTCGA237000.4542542056368538No Hit
TCACGA220140.42193890645104226No Hit
TCTTTA188190.3607008394886056No Hit
TCTAGA170170.32616218638490896No Hit
TAAAGA170000.32583635003487404No Hit
TACTCA168270.32252048600216626No Hit
TCAAGA163990.31431707671893533No Hit
TCTGGT148090.28384179456861475No Hit
TTCATG143420.27489087836471554No Hit
TCAATA136150.26095658268969474No Hit
TCCGGG134930.25861822770709153No Hit
TCATGA129900.2489773051148832No Hit
TATTGA127350.2440897598643601No Hit
GCCTCA118010.2261879274565617No Hit
TAAGGA115500.2213770495825174No Hit
ACCCGG104560.20040852211556723No Hit
TAACGA101600.1947351362561365No Hit
TCTATA100200.1920517780793787No Hit
ACCCGT91290.1749741199687274No Hit
TCCCGG86400.16560153319419482No Hit
TAAATA85740.16433652148229475No Hit
TAATGA77000.14758469972167826No Hit
GCCGGG68650.13158038488173002No Hit
ACCGGG65410.12537032738694773No Hit
TCTCCA64670.12395198092209003No Hit
TCTACA64280.12320447400142179No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)