FastQCFastQC Report
Tue 7 Jan 2025
HNFNGDRX5_n02_Rep_3-1-2_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_3-1-2_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269973803
Sequences flagged as poor quality0
Sequence length64
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA186816866.919814364358901No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG161784205.992588843888679No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA161410435.978744167262777No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC138847235.142989003270069No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC128079554.744147342325656No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG121127484.486638283196685No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT110776394.103227378694962No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC110447284.091036936646775No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT100112473.7082290536167317No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC95258613.528439016729338No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC85017973.149119249914778No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC74226712.749404170892833No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA73784772.73303443445585No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT64497612.389032168428579No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC59235802.194131406149803No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA55745712.0648562705174767No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC50453551.8688313250897162No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC50276691.8622803191019242No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC46113671.7080794316921186No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT43385081.6070107365194986No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG6395220.2368829837908384No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG6303780.23349598849781733No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG5634890.20871988086933013No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA5510950.20412906507080614No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC5507280.20399312595526167No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG5494830.20353197010007673No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA5410570.20041092653719442No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC4863040.18013006987940974No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC4783470.17718274687562927No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA4683790.17349053678367454No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA4517920.16734660732989712No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA4431650.16415111209882835No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT4383160.162355011904618No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA4260900.1578264243660708No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC4127780.15289557557553093No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG3900800.1444880931650987No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA3843170.14235344160411No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT3745610.138739757649745No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC3739500.13851343939471047No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA3687890.13660177243197186No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA3558680.13181575250840172No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC3486950.12915882805117948No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA3446190.12764905193412412No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA3324450.12313972552366496No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC3253250.12050243260084015No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC3163510.11717840638041462No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA3162270.11713247599805082No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGG3145300.1165038964910236No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGG2774300.10276182241282128No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG2736330.10135538965608452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)