FastQCFastQC Report
Tue 24 Dec 2024
HNFNGDRX5_n02_Rep_3-1-2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_3-1-2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195881
Sequences flagged as poor quality0
Sequence length6
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAA2298011.731612560687356No Hit
GCTGGT120596.156288767159653No Hit
CTAAAT119026.076138063416053No Hit
CACAAA107415.483431266942684No Hit
GCTGTT71603.655280501937401No Hit
TCTGGT68483.4960001225233692No Hit
CGTCTT54212.7674965923188055No Hit
CTGGAG48512.476503591466247No Hit
TGTGGT48382.469866908990663No Hit
TATGGA40012.042566660370327No Hit
TATATT37821.9307640863585545No Hit
GTTGGT36891.8832862809562951No Hit
AACGGT35741.8245771667491997No Hit
TCTGTT35231.7985409508834445No Hit
AATGAA32911.6801016943960874No Hit
GTTGTT31251.5953563643232371No Hit
GGTGGT28571.4585386025188762No Hit
TATGTA24891.2706694370561717No Hit
TATTTT23281.1884766771662387No Hit
CATGAA22981.1731612560687357No Hit
CTACAT22201.1333411612152278No Hit
CTTGAT21601.1027103190202214No Hit
GGTGTT20151.0286857837156234No Hit
TATTGT20121.027154241605873No Hit
CTTGAA18480.9434299396061895No Hit
TATACT18160.9270934904355195No Hit
TATAGT17430.8898259657649288No Hit
CATGGT17180.8770631148503427No Hit
CTTCTT16910.86327923586259No Hit
CGTATT16470.8408166182529188No Hit
GATATT16440.8392850761431685No Hit
CTTATT16240.8290747954114999No Hit
CTTAAT16120.8229486269724986No Hit
CTGGAT12460.6361004895829611No Hit
CGAAAT12090.617211470229374No Hit
CTTGAG12070.6161904421562071No Hit
GATGGA11950.6100642737172058No Hit
AATGTA10570.5396133366686917No Hit
CGGGGT9870.5038773541078512No Hit
CAAATT9570.4885619330103481No Hit
CTCAAT8920.4553785206324248No Hit
GTTGAA8840.4512944083397573No Hit
CGTAAT7960.406369173120415No Hit
CACCGT7520.38390655551074376No Hit
CACAAC7270.37114370459615786No Hit
GATTTT7190.3670595923034904No Hit
AAAGGT7130.36399650808398976No Hit
GATACT6100.31141356231589584No Hit
TATCAT5820.2971191692915597No Hit
TATTCT5800.29609814121839284No Hit
GCTGAA5690.290482486815975No Hit
CAAAAT5660.2889509447062247No Hit
CGTCAT5550.28333529030380694No Hit
AAACAT5540.28282477626722347No Hit
CGTGTT5490.2802722060843063No Hit
CGACAT5400.27567757975505536No Hit
TATGCA5360.2736355236087216No Hit
GATGCA5280.26955141131605415No Hit
ATTGAA5250.26801986920630383No Hit
CAAAAC5220.2664883270965535No Hit
CAACTT5150.26291472884046946No Hit
TATGAA5010.2557675323283014No Hit
GATCTT4950.25270444810880077No Hit
TCTGAA4750.24249416737713203No Hit
AACGCT4590.23432594279179705No Hit
TACGGT4530.23126285857229648No Hit
CTGGAA4500.22973131646254613No Hit
TCTGAG4280.21850000765771058No Hit
ATTAAT4190.2139053813284596No Hit
CAACAA3940.20114253041387375No Hit
ATTGAT3930.2006320163772903No Hit
AATGAT3890.19858996023095657No Hit
CTTCAT3890.19858996023095657No Hit
CGTGGT3880.19807944619437312No Hit
CACCCT3770.19246379179195533No Hit
GATGTT3680.1878691654627044No Hit
CAACCT3530.18021145491395285No Hit
GATAGT3440.17561682858470196No Hit
CAGGAA3390.17306425840178477No Hit
TTTGAA3160.16132243556036574No Hit
CACTAA3120.159280379414032No Hit
TAAAAT3090.15774883730428169No Hit
TTTGGT2900.14804907060919642No Hit
AAAGCT2900.14804907060919642No Hit
GCTGCT2880.14702804253602952No Hit
AATCAT2870.1465175284994461No Hit
TAACAT2830.14447547235311234No Hit
CCTATT2790.1424334162067786No Hit
AAGGAA2560.13069159336535957No Hit
CCTCTT2460.12558645299952523No Hit
GTTGAT2360.12048131263369087No Hit
CAAACT2350.11997079859710742No Hit
GATTGT2330.11894977052394055No Hit
CACGGG2320.11843925648735712No Hit
CTTGAC2310.11792874245077369No Hit
CACATT2250.11486565823127307No Hit
TGTGCT2240.11435514419468965No Hit
CATGAT2240.11435514419468965No Hit
AATATT2180.11129205997518901No Hit
CTGGAC2180.11129205997518901No Hit
CAGGAG2160.11027103190202214No Hit
GTTAAT2090.1066974336459381No Hit
CCGGGT2080.10618691960935465No Hit
TTTGTT2040.10414486346302092No Hit
CATTGT2030.10363434942643748No Hit
CTTGTT2030.10363434942643748No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)