FastQCFastQC Report
Tue 24 Dec 2024
HNFNGDRX5_n02_Rep_3-1-1_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_3-1-1_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960254
Sequences flagged as poor quality0
Sequence length6
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACTA819848.537741056012264No Hit
TAACTA718207.4792711095189395No Hit
GCTGGA557955.810441820601633No Hit
TCAGGA552605.754727395043395No Hit
ACACTA425874.434972413548915No Hit
TCACTA366853.82034336748402No Hit
ACTGGA291403.034613758443079No Hit
TCACGA237262.4708045996163515No Hit
AACCGT223822.330841631485003No Hit
TCAGTA214912.2380536816300687No Hit
TCTCGA206462.1500561309820108No Hit
TAACGA181621.8913745738106793No Hit
TAAGGA171331.7842154263351155No Hit
TCTCTA165231.7206905672874053No Hit
AAACGA162351.6906985026878305No Hit
ACCCGT160801.6745569401429203No Hit
TCACCA158531.650917361448117No Hit
AAAATA134151.3970262034836618No Hit
TAACCA127041.3229832940034616No Hit
TCAGCA114251.1897893682296559No Hit
CAACTA111491.1610469729883968No Hit
GCAACA108881.1338666644450321No Hit
ACTGTA107641.1209534144091042No Hit
TCATCA103351.076277734849321No Hit
ACAGTA99951.0408704363637122No Hit
ACAATA94880.9880719059748775No Hit
ACCCGG93670.9754710732785283No Hit
ACAGGA92930.9677647789022488No Hit
GCACCA91740.9553722244322856No Hit
CTACTA86280.8985122686289253No Hit
AAACGT84740.8824748451972082No Hit
AAAGTA79710.8300928712611455No Hit
ACACGA73920.7697963247224172No Hit
GTACCA70960.7389711472172987No Hit
GAACTA68090.7090832217309171No Hit
GCAGGA66850.696169971694989No Hit
AACCGG62660.6525356832671356No Hit
GCACTA61620.6417052154950669No Hit
TCATGA61020.6354568687034888No Hit
AAACCT59300.6175449412342984No Hit
GCTTGA54390.5664126366565513No Hit
GCGGGA52590.547667596281817No Hit
ACTCTA52470.5464179269235014No Hit
GCATCA52390.5455848140179578No Hit
ACCGGT52240.5440227273200632No Hit
GCACGA44890.46748047912323193No Hit
GCTCGA43550.45352583795537427No Hit
ACACGT43150.44936027342765567No Hit
TATCGA42760.4452988480131298No Hit
TCATTA40930.42624139029881675No Hit
GTACTA40450.4212427128655543No Hit
ACCGGG39100.40718393258450364No Hit
AAAGGA38460.4005190293401538No Hit
ACTTGA34620.36052960987405414No Hit
TCTCCA29180.3038779322970797No Hit
GCCCGG29110.30314895850472895No Hit
GCTCCA29110.30314895850472895No Hit
ACATCA28820.3001289242221329No Hit
AATGGA28320.2949219685624845No Hit
TAATGA27000.28117560562101274No Hit
ACTCGA26430.2752396761690136No Hit
GCAGCA25370.26420093017055907No Hit
GAACCA24930.25961880919006847No Hit
AATCTA24810.25836913983175286No Hit
TTACTA24730.2575360269262091No Hit
ACTTTA22950.23899926477786085No Hit
ACTTCA22840.23785373453273823No Hit
CCCCGG22650.23587509138207186No Hit
TCAACA22500.23431300468417732No Hit
GTACGA22450.23379230911821247No Hit
ACTATA22020.22931432725091486No Hit
GCGTGA21350.22233700666698603No Hit
ACATTA20390.21233965180046116No Hit
ACAAGA19060.1984891497457964No Hit
GAACGA18940.1972394803874808No Hit
ACATGA18780.19557325457639332No Hit
GCAGTA18110.1885959339924645No Hit
AAACCA17960.18703384729456998No Hit
GTACGT17640.183701395672395No Hit
GCTCTA17390.18109791784257082No Hit
AAACGG16480.17162125854201074No Hit
ACCCCT16290.16964261539134437No Hit
CCACTA15450.16089492988313508No Hit
CTACAA15310.15943698229843353No Hit
AAATTA14610.1521472443749258No Hit
AAAAGA14260.14850237541317193No Hit
CAACAA14100.14683614960208444No Hit
ACAGCA13810.1438161153194884No Hit
ACACCA12090.12590418785029794No Hit
GTTCGA12050.12548763139752608No Hit
AAAAAA11820.1230924317940878No Hit
ACTGCA11440.11913514549275504No Hit
ATACTA10450.10882537328665125No Hit
ACACCT10410.10840881683387936No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)