FastQCFastQC Report
Tue 7 Jan 2025
HNFNGDRX5_n02_Rep_2-2_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_2-2_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324319863
Sequences flagged as poor quality0
Sequence length64
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA206143106.356166350501942No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC168272775.188481779791576No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA157831234.866529867768229No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC156384694.821927604230642No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG155603534.797841506241633No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG137136214.228424640152244No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC128584263.96473588791569No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT125357763.8652507694232714No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT106166043.2734979294191424No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC98171703.0270023886881083No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC97894043.0184410875876573No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA97157582.9957332585577716No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC91866852.8326001728731613No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT90500892.7904824935128936No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA85305512.630289406603505No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC78365642.4163071381169154No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC69940162.1565179311881986No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC57077001.759898375388744No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC56231011.733813324902644No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT43379141.3375418822250797No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG10087110.3110235033615564No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG8429540.2599143919840642No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC8246500.2542705810158781No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA8192090.2525929162716747No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA7735440.2385126809208106No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA7687150.23702371877235284No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG7502400.23132718207888486No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC6959550.21458907683369366No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG6827330.21051223742037656No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA6759750.20842849209023007No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA6581100.20292004131735836No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG6570480.20259258681297604No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT6421870.1980103821146471No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC6355670.19596918736981583No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA6289780.19393755108980174No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA6191600.19091029278092658No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC6135140.1891694188338998No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA5882950.18139345353633182No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC5642610.17398286826484013No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA5185150.15987765757042147No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGG5040710.1554240296407624No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA4321920.13326103310545614No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC4291460.13232183685277396No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA3897580.12017703645860262No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA3827250.11800849829540043No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGG3798730.11712911953221934No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT3782380.11662498759750647No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC3246630.10010580203038627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)