FastQCFastQC Report
Tue 24 Dec 2024
HNFNGDRX5_n02_Rep_2-2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_2-2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51639
Sequences flagged as poor quality0
Sequence length6
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTT45608.830535060709929No Hit
CACTCC35096.795251650884023No Hit
CACAAC32996.388582273088169No Hit
CGAAAT24664.77546040783129No Hit
CAAAAC18693.619357462383083No Hit
CGTAAT17963.477991440577858No Hit
CGTATT16353.1662115842677045No Hit
AGTTAA13432.6007474970468056No Hit
CACTAC13262.567826642653808No Hit
TGTTGA13062.52909622572087No Hit
CGAAAA11092.1476016189314278No Hit
CGTCAT10912.1127442436917834No Hit
CGACAT10211.9771877844264996No Hit
TGTTCT9811.8997269505606227No Hit
GCTTCC9471.8338852417746279No Hit
CTTAAA9301.8009643873816303No Hit
TGTTGT8921.7273765952090476No Hit
GGTTCT8571.6595983655764055No Hit
AGTTAT7661.4833749685315363No Hit
GGTTGT6671.2916594047134917No Hit
CACTAA6381.2355003001607312No Hit
CGTAAA6251.2103255291543213No Hit
GGTTGG5851.1328646952884447No Hit
AGTTTT5211.0089273611030423No Hit
TAATAC5050.9779430275566917No Hit
CGTCTA5010.9701969441701039No Hit
AGTTCT5000.9682604233234572No Hit
CAATCC4670.904355235384109No Hit
TGTTTA4440.85981525591123No Hit
AACTAC4430.8578787350645829No Hit
AGTTTA4210.8152752764383508No Hit
GACTAC4170.8075291930517632No Hit
GCTTGC3960.766862255272178No Hit
TGTTCA3710.7184492341060051No Hit
CGACAA3650.7068301090261236No Hit
CAATAA3600.6971475047928891No Hit
CAATAC3340.6467979627800693No Hit
GGTTCG3340.6467979627800693No Hit
CGTCAA3230.6254962334669533No Hit
AGTTCA3120.6041945041538372No Hit
TGTTTT3100.6003214624605434No Hit
GTCTAC2520.4880032533550223No Hit
CGTATA2510.4860667325083754No Hit
TACTAC2330.451209357268731No Hit
GGTTCA2310.4473363155754371No Hit
CGTGGG2290.44346327388214335No Hit
GGTTGA2110.40860589864249885No Hit
CTCAAC2040.39505025271597044No Hit
GATTCC2000.38730416932938283No Hit
CGTCCT1920.3718120025562075No Hit
CTTCAA1860.36019287747632606No Hit
GAATAC1830.3543833149363853No Hit
TCTTGC1760.3408276690098569No Hit
AGGTAT1590.30790681461685937No Hit
CGCAAT1570.3040337729235655No Hit
CGTCGT1500.2904781269970371No Hit
AGTAAT1370.2653033559906272No Hit
CCTAAA1320.2556207517573927No Hit
GGTAAA1270.2459381475241581No Hit
CGATCT1220.23625554329092352No Hit
GGTAAT1220.23625554329092352No Hit
CGTGGT1180.22850945990433588No Hit
CTCTAC1170.22657293905768897No Hit
AGATAT1150.22269989736439516No Hit
CTAAAC1120.21689033482445436No Hit
CGTTCT1070.20720773059121983No Hit
TGTTGG1070.20720773059121983No Hit
GCCTAC1030.19946164720463216No Hit
GGTCAT980.18977904297139758No Hit
AGGTAA960.18590600127810375No Hit
CGGTTT960.18590600127810375No Hit
CGTACT920.17815991789151608No Hit
AGTAAA920.17815991789151608No Hit
AGTTGT910.1762233970448692No Hit
AGATAA850.16460427196498773No Hit
TGTAAC840.16266775111834078No Hit
AGTTGG790.15298514688510623No Hit
GTTTCC760.14717558434516548No Hit
TCTTCC730.14136602180522473No Hit
GTTTGC700.13555645926528398No Hit
CGGTGT700.13555645926528398No Hit
CGTTTT690.1336199384186371No Hit
GGTAAC690.1336199384186371No Hit
CTCTAA640.12393733418540251No Hit
TGTTAT620.12006429249210868No Hit
TGTTAA620.12006429249210868No Hit
CGTGTG610.11812777164546176No Hit
CGTCTG610.11812777164546176No Hit
CGGTCT590.11425472995216794No Hit
GGTTAT570.11038168825887411No Hit
CGCTCT550.10650864656558028No Hit
CCTCAA530.10263560487228644No Hit
GATTGC530.10263560487228644No Hit
TCATAC520.10069908402563954No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)