FastQCFastQC Report
Tue 7 Jan 2025
HNFNGDRX5_n02_Rep_2-1-2_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_2-1-2_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232780680
Sequences flagged as poor quality0
Sequence length64
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA142389456.1168929483323105No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG124177505.334527762355536No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA124140155.332923247754066No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC104279254.479720997464223No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC95087844.08486821157151No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG91280303.9213005134274894No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC87969763.779083384411455No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC86888823.7326474001192884No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT82386223.539220694775872No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC77378873.324110488894525No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA76867963.3021623615843034No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT74076893.1822610879906357No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC72626553.1199560891393565No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT65578942.817198575070749No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC59528782.5572904074341563No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA56856922.4425102633087934No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC53800192.3111965305711797No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC51013082.1914653741882706No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC46197101.9845762113934886No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT44939571.9305541164326867No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG6653820.2858407321432345No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG6002290.257851725495432No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA5978290.2568207120968974No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA5883590.25275250506184616No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG5873600.25232334573470616No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA5213520.22396704056367564No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC5094360.21884805903995128No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG5060100.2173762874135431No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC5055170.21716450007792745No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA4964850.21328445298810883No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA4645510.19956596054277356No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA4448520.1911034884853846No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC4314180.18533239098708706No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG4275110.18365398709205594No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA4041810.1736316776804673No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT3890280.16712211683546932No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC3861890.1659025139027861No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA3851330.16544886800743086No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC3737140.16054339217498634No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA3634490.15613366195167055No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA3611760.15515720634547506No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC3586930.15409053706690778No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT3538340.15200316452379123No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA3444070.14795342981213044No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGG3397790.14596529230862287No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC3349370.14388522277707927No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA3058440.13138719244225938No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG3023490.12988577918064334No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGG3003680.12903476353793622No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC2979730.12800589808398188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)