FastQCFastQC Report
Tue 24 Dec 2024
HNFNGDRX5_n02_Rep_2-1-2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_2-1-2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326272
Sequences flagged as poor quality0
Sequence length6
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG5665117.363120341310317No Hit
GGTGGG117303.5951598666143587No Hit
GCGGGC109973.3705006865437426No Hit
GGTGGT109123.3444488034523343No Hit
GGTAAC99813.0591040604158493No Hit
GTACTA82362.5242742251863475No Hit
GTACTT82062.515079442918792No Hit
GGTACC80812.4767678501373087No Hit
GGGGGC75812.323521479011377No Hit
AAAGTA74932.2965501176932133No Hit
GCCGCC67382.065148097293056No Hit
GGGGGT66172.0280624754805805No Hit
TAAGTA65642.011818360141232No Hit
TCAGTA57651.766930659081993No Hit
GGGGCG48321.4809729305610042No Hit
GAACTA47131.4445002942330325No Hit
GCGGCC47061.4423548450372696No Hit
GCTGGC46041.4110925853275793No Hit
GAAATT45591.3973004119262455No Hit
GGCGCC45481.393928991761475No Hit
GGCGGG38751.187659376225971No Hit
GGGGCC36191.109197234209494No Hit
GGTCTT33161.0163299333071794No Hit
GCAGCA31320.9599352687328364No Hit
GGAGTG28660.8784081992938407No Hit
GGTGTT28510.8738108081600627No Hit
GTCGCC28300.8673744605727736No Hit
GGCGGC27430.8407095919968615No Hit
GCTGTA25780.7901382895253041No Hit
GAAACC25240.7735876814437035No Hit
GGGTCC24950.7646993919183994No Hit
GGCTCC24090.7383410160847391No Hit
GGTTCC23890.7322111612397019No Hit
GTAACC21910.6715255982738328No Hit
GCCGGC21170.648845135347195No Hit
GGTGCG20310.6224867595135347No Hit
GGGGTG18410.5642531384856806No Hit
GTTGTT18020.552299921537858No Hit
GGGGTT16740.5130688505296194No Hit
GTTGTA15590.47782218517065517No Hit
GGTGGA15450.47353128677912903No Hit
GCAATT14640.44870537465672816No Hit
GTAGTA14620.44809238917222444No Hit
GCAGTA14500.44441447626520203No Hit
GCTGCC14380.44073656335817973No Hit
GCACTA13780.4223469988230678No Hit
GGTGTA13590.4165236367202825No Hit
GGGAAC13110.40181198509219296No Hit
ACAGTA12360.37882502942330326No Hit
GGTTAC11530.35338613181639855No Hit
GGTGTG11490.35216016084739116No Hit
GAATCC11300.34633679874460577No Hit
GTCGTT11170.3423523930953315No Hit
GACGCC11170.3423523930953315No Hit
GGAACG10910.33438358179678307No Hit
GGGACC10480.3212043938799529No Hit
AGGGTT10420.31936543742644175No Hit
GTAATT10320.3163005100039231No Hit
GGACTC10300.3156875245194194No Hit
GGCGCG10080.30894468418987836No Hit
GTGGGC9990.30618624950961165No Hit
GTAGTT9420.28871616320125537No Hit
AGAGGG9310.2853447430364849No Hit
GTCGTG9270.28411877206747743No Hit
GGACCG9200.2819733228717144No Hit
GTGGCC9120.2795213809336995No Hit
GAACTT8790.269407120439388No Hit
GAAGCA8360.2562279325225578No Hit
GGCGGT8060.24703315025500194No Hit
GCACTT7850.24059680266771286No Hit
GTGGTG7700.23599941153393486No Hit
CGGGTG7670.2350799333071793No Hit
CGACTT7650.23446694782267558No Hit
GTAGGA7640.2341604550804237No Hit
GAAGTA7530.23078903491565322No Hit
AGAGGT7230.2215942526480973No Hit
GTCGGC7180.220061788936838No Hit
GGCCTT6910.21178648489603766No Hit
GTAGCA6870.21056051392703018No Hit
TGTGTA6500.19922028246371126No Hit
GGGCTT6280.19247744213417026No Hit
GTACTG6190.1897190074539035No Hit
GGTGCT6090.18665408003138484No Hit
TGTGTT6020.1845086308356218No Hit
GTACCC5930.18175019615535504No Hit
GGGTAC5820.17837877599058455No Hit
GGCCCC5690.1743943703413103No Hit
TGGGTG5670.17378138485680658No Hit
GGCACC5420.16611906630051002No Hit
GCTGTT5280.1618281679089839No Hit
GAAAAC5190.15906973322871715No Hit
GGGGTA5090.1560048058061985No Hit
GGGTTG5010.1535528638681836No Hit
GGACCA4980.152633385641428No Hit
GTGGTT4820.1477295017653982No Hit
GGACCT4680.1434386033738721No Hit
CGGGTT4480.13730874852883485No Hit
GGAACT4460.13669576304433112No Hit
TGGGTT4430.1357762848175755No Hit
GGCGTT4380.1342438211063162No Hit
GATGTA4330.1327113573950569No Hit
GTAGGT4330.1327113573950569No Hit
GGAACA4030.12351657512750097No Hit
CGAATT4030.12351657512750097No Hit
GGAGTT3870.11861269125147116No Hit
CGTGTG3840.11769321302471558No Hit
GGTGCC3820.11708022754021186No Hit
GGTGCA3790.11616074931345625No Hit
GGTCTG3780.11585425657120439No Hit
GTAAAC3770.11554776382895252No Hit
GTAATA3740.11462828560219694No Hit
GGTGGC3700.11340231463318948No Hit
AGATTT3530.1081919380149078No Hit
GAAGGA3460.10604648881914476No Hit
GGCGTG3440.10543350333464102No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)