FastQCFastQC Report
Tue 24 Dec 2024
HNFNGDRX5_n02_Rep_2-1-1_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n02_Rep_2-1-1_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129355
Sequences flagged as poor quality0
Sequence length6
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGG1757613.58741447953307No Hit
AACAAC102327.910015074794171No Hit
GCGTGG63744.9275250280236556No Hit
AACCCG61904.7852808163580836No Hit
TCGTGG58304.506976923968923No Hit
AGGGGG34432.661667504155232No Hit
GGGTGG21621.6713694870704647No Hit
TAGTGT20501.5847860538827259No Hit
CACTAA19961.5430404700243516No Hit
GACTAA19891.5376290054501178No Hit
GAATAA17561.3575045417649105No Hit
AAACCG16501.2755595067836574No Hit
TGGTGG16221.2539136484867226No Hit
AACGGG15501.1982528700088904No Hit
AGGGTG14761.1410459587955626No Hit
CAATAA14211.0985273085694407No Hit
AACATG13901.074562251169263No Hit
AGGATG12330.9531908314328785No Hit
AGGTTT12190.9423679022844111No Hit
AACACG11720.9060337830002705No Hit
GAGTCA11500.8890263229098219No Hit
GACTAC11310.874338061922616No Hit
AACAAG10490.810946619767307No Hit
TAAAAG9630.7444629121410073No Hit
AGGCTG9630.7444629121410073No Hit
AAATCA9530.7367322484635306No Hit
AACTCC8700.6725677399404739No Hit
TGGTTG8700.6725677399404739No Hit
TCGTTG8660.6694754744694832No Hit
GTGTGG8530.6594256116887635No Hit
AAAACG8410.6501488152757915No Hit
ATGATG8370.6470565498048008No Hit
AACAGG8320.6431912179660624No Hit
ATCAAG8300.6416450852305671No Hit
GACTCG8100.6261837578756136No Hit
TACTTG8040.6215453596691276No Hit
AAAAAA7900.6107224305206602No Hit
TAGTCA7740.5983533686366974No Hit
AAAATG7420.573615244868772No Hit
ATGAAT7310.5651115148235476No Hit
GTGTTG7280.5627923157203045No Hit
GCGTCG7240.5597000502493139No Hit
TTGTTG7000.5411464574233699No Hit
AAACGG6940.5365080592168838No Hit
TAGTGA6880.5318696610103977No Hit
TTGTGG6340.4901240771520235No Hit
AACAAA6320.4885779444165282No Hit
ACGTGT5910.4568822233388736No Hit
GGGTTG5350.4135905067450041No Hit
ATGGTG5340.41281744037725643No Hit
AGGTTA5340.41281744037725643No Hit
ACGTTT5330.4120443740095087No Hit
AAAAAG4900.37880252019635885No Hit
AGGTAA4710.36411425920915313No Hit
AACTAC4700.3633411928414054No Hit
ACATGG4570.3532913300606857No Hit
ATGGGG4560.35251826369293804No Hit
GAATAC4400.3401492018089753No Hit
TAGTTT4370.33783000270573227No Hit
AGGAAT4300.3324185381314986No Hit
AAACAT4290.3316454717637509No Hit
AGGTAT4290.3316454717637509No Hit
TAATAA4280.33087240539600327No Hit
ATCTTG4260.3293262726605079No Hit
GAGTCC4020.3107726798345638No Hit
ATGTTG3960.30613428162807776No Hit
GAGTGA3950.3053612152603301No Hit
ATAAAG3790.29299215337636736No Hit
AGGTTG3700.28603455606663836No Hit
CTGGAG3630.2806230914924046No Hit
AAATCC3480.26902709597618957No Hit
AGATAA3430.2651617641374512No Hit
CACTAC3410.2636156314019558No Hit
AGTTAA3400.2628425650342082No Hit
ATGCTG3200.2473812376792548No Hit
ATGAAA3180.24583510494375943No Hit
GAGTGG3140.24274283947276873No Hit
AAAAAT2880.2226431139113293No Hit
GAGTGT2860.22109698117583396No Hit
AAAAAC2850.22032391480808627No Hit
AGGTGG2790.21568551660160026No Hit
ATATAT2710.20950098565961886No Hit
ACCTGG2680.20718178655637587No Hit
AGGTCT2650.20486258745313285No Hit
TAATAC2610.20177032198214215No Hit
AAAAGG2610.20177032198214215No Hit
TAATTG2590.20022418924664684No Hit
AACTCA2580.19945112287889916No Hit
TAGTGG2570.1986780565111515No Hit
TACTAC2550.19713192377565614No Hit
TAACAG2490.1924935255691701No Hit
AGATAT2430.1878551273626841No Hit
TAGTCT2340.18089753005295506No Hit
ATGTTT2310.17857833094971204No Hit
GAGTGC2260.17471299911097368No Hit
ATGAAG2250.173939932743226No Hit
GGGTCG2240.17316686637547835No Hit
AACCTG2170.16775540180124462No Hit
AGGAAG2170.16775540180124462No Hit
GACTGG2160.16698233543349697No Hit
AGGTGT2130.16466313633025395No Hit
AGCTAA2090.16157087085926328No Hit
CTGTTG2040.15770553902052492No Hit
ATCTGG1950.1507479417107959No Hit
AACGCG1930.14920180897530053No Hit
AGGTCA1900.14688260987205753No Hit
AACTGT1890.14610954350430985No Hit
AACTTT1880.14533647713656217No Hit
GAAAAG1870.1445634107688145No Hit
GTGTCG1850.14301727803331918No Hit
TACTGG1780.13760581345908546No Hit
TACTAA1750.13528661435584247No Hit
AAACAG1660.1283290170461134No Hit
CGGTTG1640.12678288431061807No Hit
TCGGAG1560.1205983533686367No Hit
AGCTAT1550.11982528700088904No Hit
AAGTAT1540.11905222063314136No Hit
ACCTTG1540.11905222063314136No Hit
AAACTG1530.11827915426539369No Hit
AAATCT1490.115186888794403No Hit
CGGTGG1490.115186888794403No Hit
CTGAAG1470.11364075605890765No Hit
ATGTGG1450.11209462332341233No Hit
AGTTAT1420.1097754242201693No Hit
ACAAAG1420.1097754242201693No Hit
ACATTG1400.10822929148467395No Hit
ATTTAA1380.10668315874917862No Hit
TACTCG1360.10513702601368327No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)