FastQCFastQC Report
Tue 7 Jan 2025
HNFNGDRX5_n01_Rep_3-2_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n01_Rep_3-2_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493441326
Sequences flagged as poor quality0
Sequence length162
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATGGTTCCGGTGGATCTGGATCAGCCATCAACGAGTTCTGCCTGAAG12383900.25097006163606167No Hit
TCGATGGTTCCGGTGGATCTGGATCAAGCAAGATGGACAGGAGGGCCCCC11308800.22918226350583373No Hit
TCGATGGTTCCGGTGGATCTGGATCAAGCGCCCAGGAGAAGATCCTGTAC9289600.18826149149899132No Hit
TCGATGGTTCCGGTGGATCTGGATCAGCCAAGCTGGGCAAGGAGCCCGTG7336380.14867785921927423No Hit
TCGATGGTTCGGGTGGATCTGGATCAGCCATCAACGAGTTCTGCCTGAAG7158070.14506425835926035No Hit
TCGATGGTTCCGGTGGATCTGGATCACTGGGCGACGGCTGTGGCCAAAGC7044040.14275334530857675No Hit
TCGATGGTTCCGGTGGATCTGGATCAAACAGCGAGCAGCTGGAGCTGATC6343360.1285534807435241No Hit
TCGATGGTTCGGGTGGATCTGGATCAAGCAAGATGGACAGGAGGGCCCCC6271070.12708846360387738No Hit
TCGATGGTTCGGGTGGATCTGGATCAAGCGCCCAGGAGAAGATCCTGTAC6270920.12708542372877782No Hit
TCGATGGTTCCGGTGGATCTGGATCACTGGAGACCTGCTGCAGCAGCGAG5696360.11544148614743305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)