FastQCFastQC Report
Tue 7 Jan 2025
HNFNGDRX5_n01_Rep_2-2_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNFNGDRX5_n01_Rep_2-2_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324319863
Sequences flagged as poor quality0
Sequence length162
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGGTTCGCGTGGATCTGGATCAGCCCAGAGCCAGACCGTGTTCCAGA11120750.3428945084378011No Hit
CGATGGTTCGCGTGGATCTGGATCAGAGAAGCACGGCTTCAACCAGCAGA6289220.19392028418561585No Hit
CGATGGTTCGGGTGGATCTGGATCAGCCCAGAGCCAGACCGTGTTCCAGA4192770.12927885332758665No Hit
CGATGGTTCGCGTGGATCTGGATCACCCCTGAGCCCCATGCTGTACAGCA3815500.11764620164507161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)