FastQCFastQC Report
Thu 11 Jan 2024
HNCYHDRX3_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCYHDRX3_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880075824
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT343827881.8287979432046564No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGAC103930990.5528021193255874No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT96249460.5119445650613291No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAA94380620.5020043276722652No Hit
GTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAAT92136610.4900685856593409No Hit
GCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGAC70629510.37567373133776333No Hit
GCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGA67372640.3583506534149231No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAG63725110.33894968057415964No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAG63497520.33773914429102303No Hit
ATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGA56887660.30258173246953046No Hit
GTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAG55528780.29535393887390365No Hit
AACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAAAAAAAAAAA51189810.2722752420223664No Hit
CAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAAAAAAAAAAAAA50002460.2659598052466633No Hit
GTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAG49637890.2640206813275846No Hit
GCTTAAACTCAGCGGGTAATCCCGCTGAGTTTAAGCATATCAAAAAAAAA47593290.25314558802602843No Hit
TGCTTAAACTCAGCGGGTAATCCCGCTGAGTTTAAGCATATCAAAAAAAA47271180.25143230606214106No Hit
GGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAG42994420.228684500120459No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAAA37523840.19958684389741935No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAAAAAAAAAAAAAAAAAAAA37337620.19859635193096337No Hit
CTTAAACTCAGCGGGTAATCCCGCTGAGTTTAAGCATATCAAAAAAAAAA36033150.1916579615567675No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAAAAAA35636300.18954714243482554No Hit
ATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACC35098160.18668481106961993No Hit
ATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAA34545280.18374407861116138No Hit
AAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGA32433810.17251330816538388No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAA31775330.1690108962328745No Hit
GACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAA30307110.16120153034849088No Hit
GATGAAAAGGACTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28081710.14936477370499926No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT27688430.14727294317891299No Hit
GCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGT27088990.1440845611341684No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGA25596190.1361444558419044No Hit
GTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAG25439940.13531337233981686No Hit
CCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATC24983960.13288804462601292No Hit
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAG24823250.13203323867644182No Hit
CCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATC24665710.13119529374896105No Hit
GGTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAA23399290.1244592888291935No Hit
CTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAA21602470.11490212109657977No Hit
CCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAA21379520.11371626466912113No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT21266660.11311596973122931No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG21154020.11251684495890843No Hit
ACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACC20513950.10911235460894901No Hit
CTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAG19771590.10516379045784699No Hit
CTTCCGATCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGGTTC19656520.10455174067490164Illumina Single End PCR Primer 1 (97% over 34bp)
GGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGAC19342210.10287994639943841No Hit
GGAAAGATGAAAAGGACTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19209780.10217555991507714No Hit
GCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTG19159810.10190977276244152No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)