FastQCFastQC Report
Thu 11 Jan 2024
HNCYHDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCYHDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880075824
Sequences flagged as poor quality0
Sequence length10
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTCAGT98204143852.23413999923867No Hit
CCGGGGCCGT32269765817.164076782469174No Hit
GGGGGGCCGG1876482089.980885111365593No Hit
CCGGTTCAGT1148962816.111257829779955No Hit
CCGGGGCCGG691306673.6770148372483944No Hit
GGGGGGCCGT592543653.1517008114030194No Hit
GGGGGGGGGG277778601.4774861548349976No Hit
GGGGTTCAGT268274041.4269320235671517No Hit
GAGGTTCAGT127200050.6765687233261289No Hit
AGATCTCGGT119816380.6372954668662343Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGCAGT74441600.39594998802558934No Hit
GGGGGGCGGG29358110.1561538615902121No Hit
GGGGGTCAGT29084410.15469806924127547No Hit
CGTTCGCGGT23930260.12728348343465534No Hit
CCGGGGCAGT23386620.12439189793017624No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)