FastQCFastQC Report
Thu 11 Jan 2024
HNCYHDRX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCYHDRX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880075824
Sequences flagged as poor quality0
Sequence length10
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGTAGTT384904942.047284131238315No Hit
GGGGGGGGGG343008881.8244417359201146No Hit
CCGTATGATT339137891.8038521939953418No Hit
CTACTAATAA337379551.794499698858954No Hit
TAAGTCCTGA327289751.740832714415033No Hit
ACTATTCATT312693251.663194888250422No Hit
TTGATACGAT309661621.647069847114847No Hit
CCAGATACGG308629391.641579483445344No Hit
GTAGCAGCTA305531501.62510200971554No Hit
TCGCCGCCGG304243381.6182505839190027No Hit
CGTTACCGCT299749101.5943458033637263No Hit
TTGCGAGCAT297903841.5845309864481294No Hit
AATATTACTT296280531.5758967070255778No Hit
TTCGAATATT293426411.5607158299377186No Hit
CGTCGAGCGC288284811.533367996757986No Hit
TGGACGACTA273163941.4529410809550412No Hit
GACTGGACCA267367511.4221102499534082No Hit
TCTTAGCCTG249728121.3282874914517278No Hit
CCTCAGCTCC245703951.3068831951535163No Hit
ATTCTTAGCT244877451.302487095860874No Hit
AATACGATAA244274071.2992777572145409No Hit
CGAATGGAGC242858671.291749337445871No Hit
TATAATAGTT242479131.2897305890786244No Hit
TCTATCGCTG241821091.2862305174772568No Hit
TGCGACCTCT240496631.2791858016041378No Hit
AGGTCAGCTT237034841.2607727676413119No Hit
TATTAGACTT236637591.2586598209456046No Hit
CAGCTAGCGG236263291.2566689437946839No Hit
GCGTTCCGTT222593201.1839586316599537No Hit
ACGGCGTTAA218363681.1614620921799588No Hit
GCTAACGGAT217513261.156938763976149No Hit
ATTATGCAAG215342751.1453939636426067No Hit
CATTATTATT215115261.1441839592529115No Hit
GAGCGCGATG214894991.1430123575696807No Hit
CTCGTCGTAG214860001.1428262480545572No Hit
TCTTGAGGTT211726511.1261594202596374No Hit
CTAATTGCGA210599131.1201629599807033No Hit
GCGCTATGGT209250431.1129893131374045No Hit
CTATATAGCC207931151.105972149344547No Hit
TCAATCTCCT204320221.0867658495033123No Hit
CTAACTAGGT200239371.0650600760025517No Hit
TAGTAAGCCG191268111.0173425324573504No Hit
GCATCGTATG190789961.0147992839676023No Hit
GGCTTACTCC190192311.0116204228154577No Hit
AGAGGAGAAT189666301.008822610124686No Hit
AAGTAGCTCA188609211.0032000177456672No Hit
TTGGCAAGCC188332801.0017298110844703No Hit
AGACCATATC186106520.989888373778695No Hit
ACCAGGCGCA182247410.9693620207947528No Hit
ATACCTCGAC181203220.9638080426696662No Hit
AGTCCGCTGC179566790.9551039788276113No Hit
ATAAGGACTC179496190.9547284620580281No Hit
ATTGGCAGAT179087060.9525523264214901No Hit
TGGTTGCCGT177122980.9421055137188978No Hit
AAGTACGCGA175823160.935191856389724No Hit
GATCTTCGCA175037950.9310153758990094No Hit
TAGGTAACTT174912340.9303472645473474No Hit
GTACTCCTAT174220560.9266677320988731No Hit
CTAAGCCTTG172679210.9184693925408404No Hit
TTATTGAGGC172551050.9177877179064242No Hit
TATTGCTGGA170070690.9045948457449023No Hit
TCCGGCTTAT169483920.901473854599175No Hit
AACGAATTCG167646850.8917025997564234No Hit
CTCAATTAGT165040540.8778398078055388No Hit
GATTCTTGAA158801220.8446532739415726No Hit
TTGCTTAGAT148072600.787588447815709No Hit
TTGATTGGCG142906350.7601095029026872No Hit
GTAGGATAAG138583940.7371188876050352No Hit
AGTTCTCATG137848250.7332058007464703No Hit
TTGGTAAGGA137776520.732824273581No Hit
GTAGGCTGCA137573590.7317449022204968No Hit
AACGCGCAGA135749210.7220411446554509No Hit
GCATATGAGC134849030.7172531462752324No Hit
CTAGCCAGCC126169570.6710876677918497No Hit
GCGGCTGCGG123928740.6591688399903599No Hit
TGATTAGGTA122729300.6527890972975992No Hit
TAGAGGCGTT121432720.6458926733159247No Hit
TCGGATTCGG121065640.6439401988714685No Hit
AGATTACGTT117421770.6245586933306579No Hit
AGTTGAATCA116207980.6181026239290655No Hit
GCTTGAAGAG109369190.5817275484523223No Hit
TGATATTGCG107845290.5736220242998029No Hit
CCGCTAAGAG107445310.5714945569131471No Hit
ATGGCGCCTG105984390.5637240192499811No Hit
TTGCCGTAGG91346930.4858683295317987No Hit
TCGTAGCATC86254070.4587797412153734No Hit
GCAAGACCGT77695410.41325679000912463No Hit
CGCGGCCATA74792810.39781805098090556No Hit
TTCCGTCGAC69991340.37227934696318926No Hit
CATAGCGACC61943800.3294750095142971No Hit
CCGCTACGAG55494520.2951717121809019No Hit
GCTTGACGAG52286060.2781061238730125No Hit
GCGCGTTCAT47635770.253371536359908No Hit
TCCTCTGCCG22736400.12093342039592123No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)