FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28822417
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7207532.500668143133173No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA332470.11535118654344638No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG303420.10527222612871086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC70100.068.699363
GCAGGTA73250.064.630052
CAATTCG118050.045.184959
GACAGTA85550.042.943127
CGAAAGC41800.040.7137033
ACTGATC127150.039.9547468
CCTTATA158250.038.4237672
CCCTTAT167600.036.3430821
GGATAGC101000.036.2202875
TTTAAAC160400.034.6290053
TATATTC108550.032.4231723
AGGTCGT75350.032.017996
GGTCGTT74400.031.8478767
ATATAGT96000.027.7306631
TATACTG294150.025.7599335
ACAAGGT156750.025.7485797
AGTAGAA250450.025.4182281
TCTAAGG77150.024.7703632
TACTGGA272400.024.2335417
ACAAGGC150800.023.1158818
Produced by FastQC (version 0.11.5)