FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAATGAGAAGACAGATAATGCAAATCAGATTTCATCTAAATATGTA15.0No Hit
GGGTCTTGTGAATGAGTCAGCATCTTTCTCACAAGATTTGCCTGTGTCGC15.0No Hit
CACACCCTCTGATCAGAAGCAGATCCGGGATGGAGCCAGCCAGGATATAG15.0No Hit
GTATGGAAGCTTGCGTAAGTGCCGTGCTAAAGGTTAAGTGACGTGATGTT15.0No Hit
GTTCTCGCGTTAACGCCCCTGCTGGGCTCCGGGCGGTTAGGCTCGCCCCG15.0No Hit
GTGGAACTGACTCCACTGACGTAGAAGACCCGTTGCCTCGAGTTAGGGTT15.0No Hit
GGACAAGGGTATGTACTGGGGACGGCGACTTTTAGAAGTAGGTATTGATG15.0No Hit
GGACTGGAGACAGGGCGGGCAGTAATACTGGACCAAGGAACATAACCAGG15.0No Hit
CTATATATCCAGAGTTAATTTGGTGTAGTGATTTAGATGCCTTTGCCTTC15.0No Hit
GCGCAGAACTGGAGAGTAGTATGGAAGCTTCCTGGAGGACATTAAAGGAA15.0No Hit
CAATTGTACCCCACATTCTACACCGTAGAACCATATCCACTTTGCAGGAA15.0No Hit
GGGGGGCGGGGGGGTGGGGGGGGGGGGTTGTGGGTGGGGGGGGGGGTGGG15.0No Hit
GACTTACACTGCCCCTTGGCTGCCCGCTCACTGCATGACCTCAAGCAAGA15.0No Hit
CACGGGAGTGGGTTAGGCCCCAGTCCTTGAGCTGCTCCGCCGCGCGCACA15.0No Hit
CTGAAGGTCTAATGAATACCTAATGAAGGGAGTGAACATAAATACGGCCT15.0No Hit
GCTTGATACCATTAAAATCATTCTGAACAGCTGTGAACTAAACCTGAGAT15.0No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG15.0No Hit
GGGTACTTGGGCTCTGGCTGGCCCGGTAAAAACCCCCAGATAAAACAGTT15.0No Hit
TGTTAAATCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAAT15.0No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)