FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_H11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_H11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG130.41827541827541825No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC130.41827541827541825No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG100.32175032175032175No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT100.32175032175032175No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA90.28957528957528955No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA90.28957528957528955No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA80.2574002574002574No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG80.2574002574002574No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG80.2574002574002574No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA70.22522522522522523No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT70.22522522522522523No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA60.19305019305019305No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA60.19305019305019305No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT60.19305019305019305No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT60.19305019305019305No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA60.19305019305019305No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA60.19305019305019305No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC60.19305019305019305No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA50.16087516087516088No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT50.16087516087516088No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA50.16087516087516088No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC50.16087516087516088No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC50.16087516087516088No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT50.16087516087516088No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG50.16087516087516088No Hit
CACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTG50.16087516087516088No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG50.16087516087516088No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC50.16087516087516088No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG50.16087516087516088No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA50.16087516087516088No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT50.16087516087516088No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC50.16087516087516088No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT50.16087516087516088No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG50.16087516087516088No Hit
GGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGA50.16087516087516088No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC50.16087516087516088No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT50.16087516087516088No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA50.16087516087516088No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG40.1287001287001287No Hit
ATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTA40.1287001287001287No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.1287001287001287No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA40.1287001287001287No Hit
GTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAG40.1287001287001287No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC40.1287001287001287No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC40.1287001287001287No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC40.1287001287001287No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC40.1287001287001287No Hit
GCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGA40.1287001287001287No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.1287001287001287No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA40.1287001287001287No Hit
ATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTT40.1287001287001287No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG40.1287001287001287No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG40.1287001287001287No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC40.1287001287001287No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG40.1287001287001287No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT40.1287001287001287No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC40.1287001287001287No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG40.1287001287001287No Hit
GTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCT40.1287001287001287No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC40.1287001287001287No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA40.1287001287001287No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA40.1287001287001287No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG40.1287001287001287No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG40.1287001287001287No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT40.1287001287001287No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG40.1287001287001287No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC40.1287001287001287No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC40.1287001287001287No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC40.1287001287001287No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC40.1287001287001287No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.1287001287001287No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA40.1287001287001287No Hit
ACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATT40.1287001287001287No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.1287001287001287No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG40.1287001287001287No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC40.1287001287001287No Hit
GACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTT40.1287001287001287No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG40.1287001287001287No Hit
GTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATG40.1287001287001287No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)