FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGCTAGAGTCCCGTGTTCGTGTGCATTACAACCCGACGGCCCCTT18.333333333333332No Hit
AGTAAGTATGCTAGAGTCCCGTTTTAGTTTCATTACCAACACTACGTCCC18.333333333333332No Hit
CTAACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTAT18.333333333333332No Hit
CCCAATTACAGCAGACAAGAGAATAATGATTCCAGAGAGGAATGAACAAG18.333333333333332No Hit
GTTCTAGAGGATATGGCTTTCCTTGAAGAATCCCACACACGAATAATCAC18.333333333333332No Hit
GCTCGAATCGACCGTGGGTGTCTTTCAACCAGAATCTGGAATATCAGATA18.333333333333332No Hit
GAATGAAGAAAAACCAAGGTATCATAAATCAGGCAAGCCTCGATAGCTGA18.333333333333332No Hit
CCACAGGACAGTGTGCATGCCCACGGCTGTCCACAGCATGACACTGTCCC18.333333333333332No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT18.333333333333332No Hit
GATAGCAAATAGTAGCATTGCCACAACTACTTCAGAGCATGTGTGAGGAA18.333333333333332No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCAA18.333333333333332No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)