FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_H06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_H06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG60.46547711404189296No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.23273855702094648No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT30.23273855702094648No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG30.23273855702094648No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.23273855702094648No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG30.23273855702094648No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.23273855702094648No Hit
ACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACA20.1551590380139643No Hit
CATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGT20.1551590380139643No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20.1551590380139643No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20.1551590380139643No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA20.1551590380139643No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC20.1551590380139643No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT20.1551590380139643No Hit
ATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATT20.1551590380139643No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG20.1551590380139643No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA20.1551590380139643No Hit
GCATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACT20.1551590380139643No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20.1551590380139643No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA20.1551590380139643No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATAT20.1551590380139643No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT20.1551590380139643No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG20.1551590380139643No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT20.1551590380139643No Hit
AGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGAAGTGCTGCAA20.1551590380139643No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.1551590380139643No Hit
CTTTAGAGCCTATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCA20.1551590380139643No Hit
GCATAGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCT20.1551590380139643No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT20.1551590380139643No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.1551590380139643No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.1551590380139643No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC20.1551590380139643No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.1551590380139643No Hit
ATCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGG20.1551590380139643No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA20.1551590380139643No Hit
CAAGCCAAATTCATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG20.1551590380139643No Hit
GCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTGTTCAGT20.1551590380139643No Hit
CTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGG20.1551590380139643No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC20.1551590380139643No Hit
CTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTCTTCTAA20.1551590380139643No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG20.1551590380139643No Hit
AAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACATGAGAA20.1551590380139643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATA100.0062713427144.999982
TTCATAT100.0062713427144.999983
AGTAAGG100.0062713427144.99998145
GCTTCAT100.0062713427144.999981
Produced by FastQC (version 0.11.5)