FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTACAGGGAGCATCTGGAGATTGGAGATTCCTTGAGATGGGGGCCTC13.125No Hit
GTAAAGTGAAGACAGTAATGCCTTTCTTCCCTTGCAGGATTTTGCATGGA13.125No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGA13.125No Hit
GGCTGACACACGGAGGAAAGGATTCTGGTCTTCACCCAGCCACAGACTCA13.125No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT13.125No Hit
CTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCT13.125No Hit
ACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGG13.125No Hit
CATAATNGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13.125No Hit
ATCCAGAACCTACAAGGTGCAAAGAAGAGCAGGCCGGCCAACTGCGGCCA13.125No Hit
GAGATATTGTCGAGAACCGGTCCAGAGCACGCAACAGCACTGCCCGATAC13.125No Hit
CTTTAAGGTGGTCCCGAGTGGGGTGCAAGAAAGCCATCTCGTTCCTAACA13.125No Hit
GGGGAGGATACTGTGGGTGATGGGTCTTTGCAAACAAACCTCAGACTGAA13.125No Hit
GAATTCATCTGACACTTCCTGCAGTCTGCTTAAAATGTTATATAATTGAG13.125No Hit
GTAGCGCCCTACGCAGGTCGTGTTCTTGTCCACAGCCAGATGAAGGGGGC13.125No Hit
AGAAAAAGGATAGTAAGAGACAACATGACCAAGAAGAAGCTCACGCAAAG13.125No Hit
GTCTTTGCCTGTCTCCTGAGGGGTCTTACTTTATGATTCCCTCCCATGGC13.125No Hit
AACAACCCCGAGATGAAGAACTGAGGTAAGCTCATGAGTTAGCCACTTAA13.125No Hit
GTGCAAACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTCGGGGC13.125No Hit
TATCAGCAGAGTTCACAAATACATTGAAAGTGGCTTTTTCTCGGTTTATG13.125No Hit
GTACAGACTCGAAAACCCACCTGGCATCACTACGACAACTAGACACCAAG13.125No Hit
AATAAATACAATACTTCAAAATAAAAACTAAGAACTGTATCTTATACACA13.125No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACAAAAACTACGTCCCCATGC13.125No Hit
GATGTGGGGGGGCGTGTCCTGTGTGGGCCGGTTCGGTGGGGGCTGTGGCG13.125No Hit
GCTCCAGAGAGGAAGGAACACTCCAGATTCAGTCCTGAGTCTGTGGCAGG13.125No Hit
CTAGTGTACATAAAAACGGCCTAGCTCAATGAATCATGTGAACACAGGCA13.125No Hit
ATTTATGGACATCAGAATTCTTATGCTCGCTCTCTGGCAGTCAGTAAGTA13.125No Hit
GCCTGATAGAATAGTTAATATGGGTGATTTTACCTGGCCACATGACAAGC13.125No Hit
ATGTCAGCTGATTCATTTACTCCAGACACTCCAAACCTCGGTAGCTCCAT13.125No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG13.125No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTGAAAACAAAAACTTCTTTCCCTT13.125No Hit
GCAGTGGGCTTTGGGGGTGGTGCCGTCGGGTGCCCGGTGCACCTGGGCTC13.125No Hit
GAGAGGAGGAAGCAGGCTCCCTGCCGAGCAAAGAGCCCGATGTGGGGCTC13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)