FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGTAACATGGTGGAATAGGAATCGCCCAACAACAAGTACAGTTCATT13.4482758620689653No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCCTTACCACAGCAT13.4482758620689653No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGTTAATTGCAGCCTACTC13.4482758620689653No Hit
GTATGACAGTGATGCGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA13.4482758620689653No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT13.4482758620689653No Hit
GTAATATATGTGGACTTACCTCCGTGTTACTCCAATTTCAATGAACCGGT13.4482758620689653No Hit
GACTAAAGAAAAACCTCTCATTAGGAACAGTTCGACAGAAGATGAGAGAA13.4482758620689653No Hit
GCAAATAGTAGCATTGCCACAACAAATTCAGAGCATGTGTGAGGAAGGAG13.4482758620689653No Hit
CAATAGTACGTTCCCTCTTTGATATCTAACCCTTAAAAATCGGTCAATAC13.4482758620689653No Hit
GGTGTTGCGATAGCCATTCTTTTTAACTTGCCTCTCTCTGCATCTTTGGT13.4482758620689653No Hit
CCACTATATGATGAAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA13.4482758620689653No Hit
GGATGACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGCCAAAATAAG13.4482758620689653No Hit
GATTTATGCCTTTCTCAAGTGGTTTGACTATGTTAGGCTCTTTCCAGCCA13.4482758620689653No Hit
CTGGTGACCCGAATGCACTATTGAAGCACCGATATGAGATAATTGAAGGA13.4482758620689653No Hit
GCACAAAGCTATTCCACTGGTGCACATGCCACTTGCATGTGGCACATAAA13.4482758620689653No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC13.4482758620689653No Hit
ATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTT13.4482758620689653No Hit
GAACAAGGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCT13.4482758620689653No Hit
AACAAACGATGCAGGATCAGACCGAGTGATGGTATCACCTCTGGCCTTAA13.4482758620689653No Hit
GTATTTGTGTTCGAAGGTTCCGTCTTTTCCTCTCGAACATTTCCCCGGTC13.4482758620689653No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC13.4482758620689653No Hit
AGTAAACAGAACACCCCAATACTCAGAAAAGGGAAAGTGGACGACAAACA13.4482758620689653No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13.4482758620689653No Hit
GAACATGGCCCTCGCACCTTGGCATATCGAACTCCTCAAGCGCAAGTCTA13.4482758620689653No Hit
CAACGAAGGTTCAAGCTTCCAGCTTCACCATAAGTGCCTGAACAATGAGA13.4482758620689653No Hit
GGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13.4482758620689653No Hit
GAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGC13.4482758620689653No Hit
GGATTATTTGAGAATTCCAAGCATGGAACAATGGATGTAGTTCCACAACC13.4482758620689653No Hit
CCGATTGAACATATCGCAAGCATGAGGATGAACTATTTTACCGCAGAAGT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)