FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCAACTCAATCCGTCGGAATCAGGAGCTCGGGGAGCCAGTGAGGCAGG14.0No Hit
GGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAA14.0No Hit
CACCAAAGCGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAA14.0No Hit
AGATAAGATGTGGGAGGAAGGATAACAGACTATCATAAGAACCATGCCCA14.0No Hit
AGACAGAGAGACAGAGAGAGGGAGTTGTTCGAAAACGCTGCTTTGAGCCA14.0No Hit
CCCTCATCTCCCTCACATCTCTGGGTTCAGACCTAGTTTGCTGCAACATG14.0No Hit
GAAATCTCCTACTTGACTTGGTTTACCAATATTGTGCCTTTGGTCCTGGT14.0No Hit
GTCTGGTGTGCTGCACCCGTACAGCGGGATGCAGAGCTTCTAGTAAGTTA14.0No Hit
CTCCAGGACGAGCAAAGCGATGGCCGCCAGTCTGTTTAGGCCCTGGCAGT14.0No Hit
ATAAGGAACATTCCCAACACATTGACGAAATAAAAAATTCCCCAGGACTA14.0No Hit
GATAAATCTCTAGCTATTACTGCAAAGCCCGAGAGTTGGGCTTAAATTTG14.0No Hit
GGGTAAGGGAGGTCGTTCTCGATACCGGACCACGTCTTCAGCCAACAATC14.0No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT14.0No Hit
GCCAAAGGCAGCTGCTTAACCAACTGAGCCACCCAGGCGTCCCGATACAG14.0No Hit
GGTCAAGCTGCTGGGTCAAGTCTCCGAAGATCTTCTGCATTTCAGAGTAG14.0No Hit
GCATACAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGC14.0No Hit
ATACAGTGTTTTTTGGCCAGAGGACGGTCTGGGCCATAAATCAATATCAT14.0No Hit
GAAGAGGTACCTAACTTGGATCTGGGGAAAGGGGAGTGGATCTAGGGAAA14.0No Hit
AGGAAATGCAGCAGACATGGTGGGGACTGTGGCGGCCCCGGGGTGCACGA14.0No Hit
GAATCTTTGACATCGGAAACTTAACCCCCAAGTCAGTAAACCTGAGAGCC14.0No Hit
GAGCACACCTGCAGTGGCCCATGTTCCAACCCAACCAACTCAGCAGTGCA14.0No Hit
GGCTTGGGTCATCTCTTAGGGTCGTAAACCCAGACATTCTAGGGTGCCCT14.0No Hit
CCCTAGACTCAGTGCGCAAGCTCAGGTCGCTGAGGATATTCTGGACAAAT14.0No Hit
GGGCTGGGGCTCCGAGCCGGGCTTCGGCGGGGGGGGGGGGGGGGTGCGGG14.0No Hit
CCTCACAGAGCACTGAGCCAGCTTGATCTCACGACCCTGCGATCAGGACC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)