FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTAGGCAGATGGTACATGCAATGAGAACTATTGGGACTCATCCTAGCT110.0No Hit
TCATCCATTATCTAACATTTTAAGCAGAATTCAGCAATGTGCAGATGCAG110.0No Hit
AACCCTGCTAGGTATTTTACTTTAAACATGCCTCCATTCATATCTACTTG110.0No Hit
GCAATGACGAGAGGATCACGTGACTCGCTGCATCTGAACTCCAATTCGCT110.0No Hit
GTGCAAATCAGTGTTCAGGTCTGTTCTGGTTTAGCATGTAAGTATGTTTC110.0No Hit
CTTCATCCACGGGCCTAGAGGGGGGGGTCTCAGGCCTACGGCAGGTGACC110.0No Hit
GTCAAGGGTGGGGGCTATGTTGATTAGAGAGGTCTACTGAGCCAGCCTAG110.0No Hit
ATTTTACAGATGAGGACAAGTGAGGCTCCGGGAGTGGGAGCAGGTCCAGG110.0No Hit
CCCAAAGCAGAAGAAGCTCACCACACGGTCACAGGCAGTACAAACGGAGA110.0No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA110.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)