FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R2_G10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_G10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT21.2121212121212122No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG21.2121212121212122No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC21.2121212121212122No Hit
GAATAAGTTATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTC10.6060606060606061No Hit
CAGCCAGACCTTGGCATGGACGTCCCAGCAGATCTAAGACGTTGTCAGCC10.6060606060606061No Hit
GCCCTCGGCCCTGCCCCGGCACCTGGAGCAGTTGAGGCTCAGCCAGAACC10.6060606060606061No Hit
CTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTC10.6060606060606061No Hit
GTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAAATGCTAGCAAGC10.6060606060606061No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.6060606060606061No Hit
CTCAAATGATCTTCTCGTCTGAATTTGTGTGTCTCCCCTATGGCACCTAT10.6060606060606061No Hit
GCTCTATTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAAC10.6060606060606061No Hit
GTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT10.6060606060606061No Hit
TTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGT10.6060606060606061No Hit
CAAAAGAAGTGAACGCCAAAATTGAACCATTCTTGAGGACGACACCACGC10.6060606060606061No Hit
CCCGCTTACTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAG10.6060606060606061No Hit
AGCAGACGACACACCGAAGAGTGCTAAGTGGTGGAGAAGCCTCTGGAGAG10.6060606060606061No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT10.6060606060606061No Hit
CCGCAGGTGCTGCGGTGAAAGGAGTTGGAACAATAGCAATGGAGTTAATC10.6060606060606061No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC10.6060606060606061No Hit
ATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTACAA10.6060606060606061No Hit
GGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAACATAGT10.6060606060606061No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA10.6060606060606061No Hit
TAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAG10.6060606060606061No Hit
ATCCCACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTT10.6060606060606061No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA10.6060606060606061No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10.6060606060606061No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC10.6060606060606061No Hit
AATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGA10.6060606060606061No Hit
CCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTCACATCT10.6060606060606061No Hit
GTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGACATCCTTAGAC10.6060606060606061No Hit
TTCTAGTACTGTGCCACTATTGAATTGAATAAGTTCATTGTTGTTTAAAT10.6060606060606061No Hit
ACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGG10.6060606060606061No Hit
CAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACA10.6060606060606061No Hit
AGTTTGGCTGCCTCTAAACTTAAAAATCACAATCTAAAACAACATCCTGT10.6060606060606061No Hit
CCTCAATGCAGCCGTTCGGCTCGAATCCATCTACATAGGCTCTAAAGTTT10.6060606060606061No Hit
ATGCTAGAGTCCCGTTTTAGTTTCATTACCAACACTACGTCCCCATGCCC10.6060606060606061No Hit
ATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAATA10.6060606060606061No Hit
CACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTG10.6060606060606061No Hit
TTGCCATTTTGTTTGAGAACATTATGGGTGCCATGCTCAGNATNTTTCTG10.6060606060606061No Hit
CCCTAGTCTTGCCATTTTGTTTGAGAACATTATGGGTGCCATGCTCAGGA10.6060606060606061No Hit
CATCAAGTTCTATCCAGCTTGAATCAGTCAATTCACATGCCTTATTGAAT10.6060606060606061No Hit
GGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTTCAAAAG10.6060606060606061No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10.6060606060606061No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT10.6060606060606061No Hit
GATCATCAGTCAAGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACA10.6060606060606061No Hit
ATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGA10.6060606060606061No Hit
GCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTA10.6060606060606061No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC10.6060606060606061No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT10.6060606060606061No Hit
GATCCCACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCT10.6060606060606061No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA10.6060606060606061No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA10.6060606060606061No Hit
GATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCC10.6060606060606061No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGAT10.6060606060606061No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10.6060606060606061No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC10.6060606060606061No Hit
GGCAAACCATTTGAATGGATGTCAATCCGCCTCTACTTTTCCTAAAAATT10.6060606060606061No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT10.6060606060606061No Hit
GGATGATGGCAATGAGATAATTGGGCAAGGGGACGTAGTGTTTGTTTTTT10.6060606060606061No Hit
TCGTCAACATCCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCAT10.6060606060606061No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10.6060606060606061No Hit
ATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCC10.6060606060606061No Hit
CAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGCGATATG10.6060606060606061No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCAG10.6060606060606061No Hit
GAATTGTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT10.6060606060606061No Hit
GCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAAATGAATTCAATAAGG10.6060606060606061No Hit
GTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAAT10.6060606060606061No Hit
AAATAACGTTCCATGGGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGT10.6060606060606061No Hit
TATCTACCCTTGTTTGTTGAACAACTTCCATTGTTTCAAGGCATGAATTC10.6060606060606061No Hit
GAATTATGTTTACAAACTAAATAAGGTAGTGCATGGGCTCTGAAGAAACA10.6060606060606061No Hit
GGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAATGGAATG10.6060606060606061No Hit
TTCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCT10.6060606060606061No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA10.6060606060606061No Hit
CACTTCTTTTGACATTTGGGAAAGCTTGCCCTCAATGCAGCCGTTCGGCT10.6060606060606061No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT10.6060606060606061No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG10.6060606060606061No Hit
GTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTGTCTTTG10.6060606060606061No Hit
ATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTT10.6060606060606061No Hit
CATTAGGCAAAAACCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCA10.6060606060606061No Hit
GGTCACAAGATCCCACAATGTTATACAACAAAATGGAATTTGAACCATTT10.6060606060606061No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG10.6060606060606061No Hit
GCCTCTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGAGGAATC10.6060606060606061No Hit
CATCATCATTTCTCACTTCTCCTCCTGGAGTGTACATCTGCTCCCAGCAC10.6060606060606061No Hit
GAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAA10.6060606060606061No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT10.6060606060606061No Hit
ATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCA10.6060606060606061No Hit
TAATTGACCCGAATGCACTATTGAAGCACCGATTTGAGATAATTGAAGGA10.6060606060606061No Hit
CTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAG10.6060606060606061No Hit
CACTGGGGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCC10.6060606060606061No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT10.6060606060606061No Hit
GAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGG10.6060606060606061No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACT10.6060606060606061No Hit
GGGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAG10.6060606060606061No Hit
CCTCTATCTTCCCTGACTGCCCCCGGGAATGCTACTGCCCCCCGGATTTC10.6060606060606061No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC10.6060606060606061No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC10.6060606060606061No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA10.6060606060606061No Hit
GTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGC10.6060606060606061No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACAAAATGGCCAAAATCAAA10.6060606060606061No Hit
GAACCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATAATTGA10.6060606060606061No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG10.6060606060606061No Hit
TCATTCCACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTC10.6060606060606061No Hit
GGCAAAGAGGCCCATCAGGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAG10.6060606060606061No Hit
GGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGT10.6060606060606061No Hit
GTTCCACCATTGAAGAACTCAGACGGCAAAAAAAATTAATTTAACTTGTA10.6060606060606061No Hit
GTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGACTAAAC10.6060606060606061No Hit
GTTCGAATCTTCATTCTTTTACTCTCGAACATGTACCCTTTCCCTAGTCT10.6060606060606061No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTG10.6060606060606061No Hit
CTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCC10.6060606060606061No Hit
CACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTG10.6060606060606061No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT10.6060606060606061No Hit
AGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGAA10.6060606060606061No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA10.6060606060606061No Hit
GTGGACGACAAACACAGAGACTGGTGCACTCCAGCTCAACCCGATTGATG10.6060606060606061No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT10.6060606060606061No Hit
ATTGGAGCCCATCCCACCAGTATATTGTCTTGGTGTATTTCTTTTGTCCA10.6060606060606061No Hit
GCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTGAAAATGAAGAGAA10.6060606060606061No Hit
ATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCA10.6060606060606061No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGTGCCATATCTATAG10.6060606060606061No Hit
GAGAAGGAGAGTTTTGAATGGGACTTTTGTTAGACTCAATAGAAAATTAG10.6060606060606061No Hit
GATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACA10.6060606060606061No Hit
GAGAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGC10.6060606060606061No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC10.6060606060606061No Hit
TAGGAAACACTCGAGGTTCACTACAATTTGTCCTTATTCCAAAATAGGTT10.6060606060606061No Hit
TACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATC10.6060606060606061No Hit
AGTGTGGCTTGGGCAATTCTCTCGTGCTCAGTCACTAGCTGGAACAGCCA10.6060606060606061No Hit
GACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAG10.6060606060606061No Hit
GGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGC10.6060606060606061No Hit
TCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATACCACA10.6060606060606061No Hit
GTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACATTCC10.6060606060606061No Hit
GGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGA10.6060606060606061No Hit
CCCTTGAGAGCAGATGTGTGAGGCCAGCCTGTGTCTGCGAGCTCCGGTTG10.6060606060606061No Hit
AGCGATTTGCAGACAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTC10.6060606060606061No Hit
AAAAAAAAGAAAGAAAGAAAGAAAGAGAAGATTGTGCATGTAGCCCATGA10.6060606060606061No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA10.6060606060606061No Hit
GACCTAGCCTGCTCTGGTGAGGGTGAGCCATCCTGGCAGAAGGCACACAT10.6060606060606061No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT10.6060606060606061No Hit
GGAGTGGAGGAAATACCAATCAACAAAAGGCATCCGCAGGCCAGATCAGT10.6060606060606061No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA10.6060606060606061No Hit
AGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAA10.6060606060606061No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10.6060606060606061No Hit
GGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAA10.6060606060606061No Hit
GTACAGTGGATTCGTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTG10.6060606060606061No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA10.6060606060606061No Hit
CACCTAGAGAAAGCCAACAAAATAATTGAAGGAAGAGACCGAATCATGGC10.6060606060606061No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCA10.6060606060606061No Hit
ATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAA10.6060606060606061No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10.6060606060606061No Hit
CGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAAT10.6060606060606061No Hit
CCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGTGGGATCTCTA10.6060606060606061No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA10.6060606060606061No Hit
TCTGGTGATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTT10.6060606060606061No Hit
CATCTTCTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCA10.6060606060606061No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.6060606060606061No Hit
ATCATGGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGGGGTAGA10.6060606060606061No Hit
CATTAGCTGAGTGTGCCAGGGAGCGGGAACAGTGTGGCTTGGGCAATTCT10.6060606060606061No Hit
CTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATAT10.6060606060606061No Hit
GTTTAAGCTGTTGCTAAACAAGGAACAGGGATACCATAATAAATAGGAAA10.6060606060606061No Hit
GTCCTCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGA10.6060606060606061No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCG10.6060606060606061No Hit
CTTGCCATTTTATTTGAGAACATTATGGGTGCCATGCTCAGGATGTTTCC10.6060606060606061No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC10.6060606060606061No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers