FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R2_G08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_G08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCACATATGGCTCTTTAGCCAATGCCTTACCATTTAATGTACCCA11.2987012987012987No Hit
CAGCACCATTAAGCAAACTGGGGTAAAAGAATGAATTCTGAATAAATTAG11.2987012987012987No Hit
TCTCATACCTGTCTCAGTTCTCTCAGTTCACCCCTTCGCCCTGAATGTCC11.2987012987012987No Hit
GTTTTATACATGCTCTTCAGGGTAACTAACTGGCCAGCCACGTTCCTCCA11.2987012987012987No Hit
CTGCTAAACACACCTTCTGTTTCTTGTGCTCTCCAAATACGTTCTCATCC11.2987012987012987No Hit
AGGTCAGTGAGACTGAGAGATGAGGAATCAGCATTATCATGGCGACCAGA11.2987012987012987No Hit
CTCCCACCACGCTTGATCAAGCCTCTGTGCCTTTGCACACAGTTCCGTCT11.2987012987012987No Hit
CTGGAAGGCTGATCCCTCTGGCCCTTTGCAGTGTTTGGACTTGAATTTGT11.2987012987012987No Hit
GCTCTTCAGTAAATCCTCAGTTACTTCAGCAGCTGCAGTAAGTGCGTTGG11.2987012987012987No Hit
ATCAGAGAGCAGAGACTTTGTCTCTCAACCTTCAGGTAATCCCAGCCCCT11.2987012987012987No Hit
GCTGATGGCGACGGTCATCTTCCTCCCCTACGTCAGCAAGGTCTCCAGCT11.2987012987012987No Hit
GTCCTGCCCTTTTCTTTCTGTTTTCTAAGCGTGCCTCCCACCTGTGGCTC11.2987012987012987No Hit
GAGGAGGACCTGGCTCAGGACGAGCAGGAGGTGAGCGGCGATGAGGCCCT11.2987012987012987No Hit
GGAACGGTGCCGGCAGCTGTGGTGGCGACGAGACCTGGAACGTGCCCTCT11.2987012987012987No Hit
GTGTCGGGTTTGTGTCGCTCTCCGTTGTATACGTCACCATCAAACAAAAA11.2987012987012987No Hit
GCCTTAGCCTGTGGGCCCCGTGAAGGCCATGGAGTCAGCACAGGCCACGG11.2987012987012987No Hit
CACTTCTTGTGCAGTGGCAGAGACTCCAACTTATCTACAAGACAGCGGAG11.2987012987012987No Hit
CTTTGCTGCTTTAAGGATTCGGGGTGCGTCCCGCCAGCGAGAGTGCTGGG11.2987012987012987No Hit
CTCTGCACTGACTCTGTTTAGGATGGGCTGACCGCGCAGCTGTGCACACA11.2987012987012987No Hit
GTGCAAACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTCGGGGC11.2987012987012987No Hit
TCTTTGGAATTTACCAAAAAGAGAAAGAGAGAGAGAGCGAAACTACTCTT11.2987012987012987No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC11.2987012987012987No Hit
GGCTTTGCACCCTCCAAGGCTCAGGCCAAATGTCACCTCCACCAAGAAGC11.2987012987012987No Hit
GTAGAGAGGTGTGACAGGAGACTAGTGCTGGATGGCCAGAGACAAGGCTG11.2987012987012987No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT11.2987012987012987No Hit
CCTCATACCTCAGCGATGCTGGAAACATGAGCTGTTTGATTGGAAACCAA11.2987012987012987No Hit
GGTGAAGGTCGGCGATCACGAGGTGAGCGGAGCTTTGTGGCCAGGTTCAT11.2987012987012987No Hit
CCCGGTAGGGAGCAGCGCCTTGGGTCCCTTTGCTGCTTTAAGGATTCGGG11.2987012987012987No Hit
GGATGCAATCTGGTCGCCATGATAATGCTGATTCCTCATCTCTCAGTCTC11.2987012987012987No Hit
GAGGAAAGCATCTGTATAACCCCAGATCTGTTCATAAGGGCCAGATATGA11.2987012987012987No Hit
GTTTGCACACCACTGCTGTATCACTTTCTGATAAGCAGTTTATGCATCCC11.2987012987012987No Hit
ATGCATTTGTTTTGCATGTATTTGCATTATGGCATGAAAACCATGGGGCA11.2987012987012987No Hit
GTTTAACTGTCACCCCGTATCTTCTTTATCAACCCAGTTCTGACTTGGGG11.2987012987012987No Hit
CTGTATGCATGTATTTTTAATTTTAATGCATTTGAATGTTGCCCTTAGCT11.2987012987012987No Hit
GCAGGGAGGCCAACGCAGGGCTTGATCCCATGACCCTGGGCTGAAGGCAG11.2987012987012987No Hit
AAACCAAGGTCACGTTTCTCTGTGAGAAAACCGGGGCGTGGCTGAGAAGC11.2987012987012987No Hit
CTGCCACTGCTCCTGCTCCTGCTCCTGCTCCTGCTCCTCCACCTGCAACA11.2987012987012987No Hit
AGACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAAC11.2987012987012987No Hit
AACTAAGGAATTTCAAAAAGAGTCCCTCAGTATGGATTTGGAGTTAAATG11.2987012987012987No Hit
GGTTCATGCTTTCTTTCTCTGTCTCTCTCTCTCACACTCTCTCAAATAAA11.2987012987012987No Hit
GCTTCAATATCCCCATGCAGTTAAAAATATACAACATTGAAGATCGAACA11.2987012987012987No Hit
GGGGTAAAAGAATGAATTCTGAATAAATTAGTCACCGAGGAAGGGGCTAG11.2987012987012987No Hit
CTCTGGTGGGGCCCAATGGGTCCGGGAAGAGCACAGTGGCTGCCTTGCTG11.2987012987012987No Hit
ATTCAGGCATGGAGGTGGCCAGCTGGCGGAAGCCCGGTCTGAGGGCAGAG11.2987012987012987No Hit
CAATTTTTAAAAGAAAAAAAAAATACAGTGACATTTCACAAAATCTCAAA11.2987012987012987No Hit
GTATCAGCTTGGTTTACTTGGGGGGGTTTTGAGAGGGTTTTTTGTTGTTT11.2987012987012987No Hit
CTCCCATAATCAGGCACCCCATTCTGTGTGACCCTGGGGATCCTGAGATC11.2987012987012987No Hit
GTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTC11.2987012987012987No Hit
GCTCCCAGCGCTCCAAGCGGTTTTCGGCCAGATGCAGGTAGCTGTGGCGA11.2987012987012987No Hit
CACGGGTGTTAACCGAGGAGCCGGTTTCATGGTGTGAGGCTTTGTGATGG11.2987012987012987No Hit
GTGTGTGAGGTCAGGATTTGTGGCCAAATGAGTGTGACTAACTCAGAGGT11.2987012987012987No Hit
AAGCAGGTTTGAGTCCCAGGACCCAGAGATCAAGTTTGTATTCTGCTGCT11.2987012987012987No Hit
CTCTCACCCCGGAGGTGCCCTAGGAGCTAATTCACATCTTGGAAGTGCTC11.2987012987012987No Hit
TGTGAGGCTTTGTGATGGGGATGGTAGGAACCCGCACCTTTGGCCGGGCG11.2987012987012987No Hit
CTCTCTCTCTCAAATAAATAAAATCTTAAAGAAAAAAAAAACCAAACAAC11.2987012987012987No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT11.2987012987012987No Hit
CATAAGGGCCAGATATGAATAAGAGGGCCACCTCTGTCTCTTGAGGTCAT11.2987012987012987No Hit
GTCCTCTCAAGAGGCGTACCCCACCCTGACGCATCAAGCTCAGAGTATTT11.2987012987012987No Hit
GTCCCCAGGTGGGGATGGGCTGGACCTGTCCGGGTCCCCTGGGGAGAGGC11.2987012987012987No Hit
TTCTTACACAAAAATAGTAGTGGTGATAATGAATGCAAAGCTTCATTTTT11.2987012987012987No Hit
ACCCTCTGTGCCGTTACTGTCTAGATTGCACGAGATTATGTGTTGAAGTA11.2987012987012987No Hit
TTCTTTCATCAGTGTTTTCTAGTTTTCAGTGTATAGATTTTTTAATTCTT11.2987012987012987No Hit
AGCTGCTTGAGCCTCAAACGCAAGGCTGGGAGCTGGGGGTGGTGGACATC11.2987012987012987No Hit
AGGGGGAGGGATGTCACCTACAACCTTCTGGAAAGGCAGGGAGCATGCCC11.2987012987012987No Hit
CTCTCTCGGAGATAGCCCAGTGCACGTATAGGAGTTCTACACCCAAGAGA11.2987012987012987No Hit
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC11.2987012987012987No Hit
GGGCAGGAGAGGGAGACAGAGGCAAGGTCTCATATTCCAGCATGAAAAGA11.2987012987012987No Hit
CATTGTAGCAAGTCTGGATTCTCATTTTTCCTCTTAGTTGCTGTTTGGTA11.2987012987012987No Hit
GTAACATACTTGGAATTAGGTAAAAGAAATTCCTAGAAACTATAACCTGC11.2987012987012987No Hit
ATCTGGTGCATTGCAAACTTCCAGTGAATGTTGGTCATTGTTTTAGTCTG11.2987012987012987No Hit
ATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGGCTGTACGGAAAGGC11.2987012987012987No Hit
GAGGAATAGAGTGGATGCCCACATCTCAATCCTGGTTCTGCCCCTTCCTA11.2987012987012987No Hit
GGCGGGAATGAGCACCCCGGCGGCCCCCCCAACCACCAGACCCACCCCTC11.2987012987012987No Hit
ATTATTACTACACCTAAGGAAAATCTTGGATTGATAGAGCACAACTAATA11.2987012987012987No Hit
GTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCCTGTGTCCC11.2987012987012987No Hit
CTACAAGACAGCGGAGGCCTTCAAGCTCAAAATGGGTCAGATGCACCTGC11.2987012987012987No Hit
ACATCCAGCTGGGGTGGTGGTGGGGGAGGAAAGAAGGGCTCCACTGAATC11.2987012987012987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAAG50.0145.05
TCTAGGA50.0145.03
GGACTTG50.0145.0145
GAAGTGG50.0145.08
AGGAAGT50.0145.06
CTCTAGG50.0145.02
CTAGGAA50.0145.04
GGAAGTG50.0145.07
AAGTGGC50.0145.09
GCTCTAG50.0145.01
Produced by FastQC (version 0.11.5)