FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG150.8147745790331342No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG140.7604562737642585No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC140.7604562737642585No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA110.5975013579576317No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA90.48886474741988045No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT80.43454644215100485No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA80.43454644215100485No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC80.43454644215100485No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG70.38022813688212925No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC70.38022813688212925No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT70.38022813688212925No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA70.38022813688212925No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG60.32590983161325365No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG60.32590983161325365No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT60.32590983161325365No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT50.27159152634437805No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC50.27159152634437805No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG50.27159152634437805No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA40.21727322107550243No Hit
ACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT40.21727322107550243No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT40.21727322107550243No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC40.21727322107550243No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA40.21727322107550243No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.21727322107550243No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC40.21727322107550243No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC40.21727322107550243No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA40.21727322107550243No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA40.21727322107550243No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC40.21727322107550243No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG40.21727322107550243No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG30.16295491580662683No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC30.16295491580662683No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA30.16295491580662683No Hit
GCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCA30.16295491580662683No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAA30.16295491580662683No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT30.16295491580662683No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.16295491580662683No Hit
GAGAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCC30.16295491580662683No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT30.16295491580662683No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC30.16295491580662683No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT30.16295491580662683No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.16295491580662683No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA30.16295491580662683No Hit
GAACAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC30.16295491580662683No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG30.16295491580662683No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA30.16295491580662683No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA30.16295491580662683No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT30.16295491580662683No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG30.16295491580662683No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG30.16295491580662683No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC30.16295491580662683No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG30.16295491580662683No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC30.16295491580662683No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG30.16295491580662683No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT30.16295491580662683No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA30.16295491580662683No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC30.16295491580662683No Hit
GTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTT20.10863661053775121No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG20.10863661053775121No Hit
GGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGG20.10863661053775121No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG20.10863661053775121No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG20.10863661053775121No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTAAGTCCCCTTGC20.10863661053775121No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20.10863661053775121No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT20.10863661053775121No Hit
AAACTAACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATG20.10863661053775121No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT20.10863661053775121No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC20.10863661053775121No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC20.10863661053775121No Hit
ATGCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAA20.10863661053775121No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC20.10863661053775121No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA20.10863661053775121No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20.10863661053775121No Hit
GCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCC20.10863661053775121No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC20.10863661053775121No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT20.10863661053775121No Hit
GAACAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCA20.10863661053775121No Hit
ATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGG20.10863661053775121No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.10863661053775121No Hit
GAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACA20.10863661053775121No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA20.10863661053775121No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.10863661053775121No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAG20.10863661053775121No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT20.10863661053775121No Hit
CCCTTATACTGGAGATCATCCATACAGCCATGGAACAGGAACAGGATACA20.10863661053775121No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACAAGG20.10863661053775121No Hit
CAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCA20.10863661053775121No Hit
GCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTT20.10863661053775121No Hit
CTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATC20.10863661053775121No Hit
GTATGCTAGAGTCCCGGTTTCGTTTCATTACCAACACTACGTCCCCTTGC20.10863661053775121No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA20.10863661053775121No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG20.10863661053775121No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTCTCTGGAAT20.10863661053775121No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT20.10863661053775121No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT20.10863661053775121No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA20.10863661053775121No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG20.10863661053775121No Hit
CATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCATCATCCA20.10863661053775121No Hit
GAACTTTGACCGCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGC20.10863661053775121No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA20.10863661053775121No Hit
GAAAACAAACCTGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAA20.10863661053775121No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT20.10863661053775121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.10863661053775121No Hit
GCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGG20.10863661053775121No Hit
AGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTC20.10863661053775121No Hit
GATGGATGCTCTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGG20.10863661053775121No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC20.10863661053775121No Hit
ATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATATGGGGAA20.10863661053775121No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC20.10863661053775121No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC20.10863661053775121No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG20.10863661053775121No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA20.10863661053775121No Hit
CAAGTACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGT20.10863661053775121No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC20.10863661053775121No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGG20.10863661053775121No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA20.10863661053775121No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA20.10863661053775121No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG20.10863661053775121No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA20.10863661053775121No Hit
AGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAA20.10863661053775121No Hit
TTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAA20.10863661053775121No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA20.10863661053775121No Hit
ACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC20.10863661053775121No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20.10863661053775121No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG20.10863661053775121No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT20.10863661053775121No Hit
CAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGG20.10863661053775121No Hit
AAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAG20.10863661053775121No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT20.10863661053775121No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTGC20.10863661053775121No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA20.10863661053775121No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG20.10863661053775121No Hit
CATCAAAGTCTACTTTTTCTGGTGCCATATTTTCACCGAGTGCCCACTTC20.10863661053775121No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC20.10863661053775121No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGGCC20.10863661053775121No Hit
GTTAAGTTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC20.10863661053775121No Hit
GTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGG20.10863661053775121No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA20.10863661053775121No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG20.10863661053775121No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG20.10863661053775121No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT20.10863661053775121No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC20.10863661053775121No Hit
GAGAAATCCTCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTG20.10863661053775121No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA20.10863661053775121No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT20.10863661053775121No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)