FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCCCATATTGCCATAATCAAAAAGTAACAAACAGTANGGAAATATAA13.4482758620689653No Hit
GGTGGGCACAGTCGCACACGGGCTTCCGCGCCTGCCAGGGAGCAGGGCCT13.4482758620689653No Hit
CCCTTCTACAGCTTCCCAATGCCCACACGGTCAAAGCTAAGCTCCACTAC13.4482758620689653No Hit
CCCTTACACTGGAGATCCTCCATACAGCCATGGAAGAGGAACATGATACA13.4482758620689653No Hit
GTACTCACTGGTCGGGATAGACCCATTCAAATTACTCCAAAACAGCCAAG13.4482758620689653No Hit
ATTTTGAAGTCGTACGCATATTTATCACCAAATGATACCTGGTGTACTGC13.4482758620689653No Hit
ATTAACCTCAGTAGGTGAGCAACATCACCCGGTAAATAAATAATACAAGC13.4482758620689653No Hit
GACACTTAACAGACTGAGCCACCTGGGCGCCCATCTGCTGTTGAATCATA13.4482758620689653No Hit
GGCTATGGACGGACTGAAGCTGTTGACTGGTGGTGCTCACAGACAGTCAG13.4482758620689653No Hit
ATTGAAGTAAGAGAACGAATCATGGCCGGGACAGTGGTGAACAGTATATG13.4482758620689653No Hit
CTATACACATAGTCCCTAAAACACCTTACGAGGCTAATTCATCGATATCC13.4482758620689653No Hit
AAACAAGGTCCTTTTTAAACAATTCGCCACTAATTGATGGCCAACCGAAT13.4482758620689653No Hit
TCTCATGACTGTATCTCCAAGCTAATCTCTAAATTATTTAACAAAGTCGC13.4482758620689653No Hit
GACCACTGTGGTCCATATGGCCATTATCAAAAAGTACACATCAGGAAGGC13.4482758620689653No Hit
CTTTGGGACAAAATCATAACCAAACTGGTAGTCAAGAGGCTCTGATCCTA13.4482758620689653No Hit
AAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCAT13.4482758620689653No Hit
AAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAAT13.4482758620689653No Hit
GACCCCTGTTGACCATATTGCCATAATCAACAAGTACACATCATGAAGGC13.4482758620689653No Hit
AGAATGTGCTCTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGC13.4482758620689653No Hit
AAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTC13.4482758620689653No Hit
ATATGGAGACAATAAAAGAACTGAGAGATCTAAAGTCGCAGTAAAGCACA13.4482758620689653No Hit
TCCCCACCCATAGCTGTGTGACTTGCAGTTATCTTTCTGTCCCGATTCTT13.4482758620689653No Hit
GCCTTTCTGAGAATAGCTGTTGCTCTTCTCCCAACCATTGTGAATTCTTC13.4482758620689653No Hit
CTCTACATATTCTGAAGAGACTGAAGCAGGACTCTCATCATTTCCATGCC13.4482758620689653No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT13.4482758620689653No Hit
GGGTAGGTAGTCGCGCGATGAGGGAGGTCGAGGGGATGGCGGCGGAGGGC13.4482758620689653No Hit
GCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAG13.4482758620689653No Hit
TCCCTGCTGAGCCCGGAGCCCCATGTGGGTAACCCGCCACCCACCCATAG13.4482758620689653No Hit
TTGTTGGACTGGCCAGTGGCCAAAGCCTGGGCTAGCAAAGGTACTTTTGT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)