FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCA10.7751937984496124No Hit
CCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAA10.7751937984496124No Hit
CATATACAACAGGATGGGAACAGTGCCCACAGAAGCTGCTTTTGGTCTAG10.7751937984496124No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCTAGGAACTGAGAAGTT10.7751937984496124No Hit
GCAGTGGGACCCTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGCGATAT10.7751937984496124No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC10.7751937984496124No Hit
CATCAGGCTGACCACTTGGCTGTTTTGGAGTAATTTGAATGGGTCTATCC10.7751937984496124No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG10.7751937984496124No Hit
ATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGACGTTTTATTAT10.7751937984496124No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.7751937984496124No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGC10.7751937984496124No Hit
CCATCATTCTTATAACTTCTGTTCGCATGTCGGATGTCCGTCCTTCATTG10.7751937984496124No Hit
ATTGAATCCATCGACAATGTGATGGGAATGATCGGAATACTGCCCGACAT10.7751937984496124No Hit
CCCGGGAACTGGGCAGCGGGTGCTGGGCGAGAAGGGCGAGGAGGGGGGGA10.7751937984496124No Hit
GGGGGGGGCAGGCGGGGGGTTGCGGGTGAGTGCATGTGCGTTAGGCTCAT10.7751937984496124No Hit
CTCTAATGCTAGGTTCAACCCTTCCCAGCAGGGCGGGTGCCGCAGGTGCG10.7751937984496124No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT10.7751937984496124No Hit
CTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTT10.7751937984496124No Hit
GCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAA10.7751937984496124No Hit
CACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAAC10.7751937984496124No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC10.7751937984496124No Hit
TAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCATGT10.7751937984496124No Hit
TATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATT10.7751937984496124No Hit
ATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAGAA10.7751937984496124No Hit
GAAGGCTATATTTTCAGTATCTATAACTATTCAATCCACCTAACAAGGGG10.7751937984496124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.7751937984496124No Hit
AATCTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCACTTTCTTTC10.7751937984496124No Hit
GTATAGCATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT10.7751937984496124No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGAT10.7751937984496124No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG10.7751937984496124No Hit
TTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC10.7751937984496124No Hit
ACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGT10.7751937984496124No Hit
GGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.7751937984496124No Hit
TTTGGTCTTGGATTTGGTGCTCAGTTTGTGGGGTTCATAGTCAGTTGTGC10.7751937984496124No Hit
GTGTCGGGTGGTTTGGGGGGTCAGGCGGAGGGGTGCACAGCGAGGGGAGA10.7751937984496124No Hit
AAACAGAACGGGGAAGCTGCTCCTTGGAGTGGCGGGAAATGCCCAGAATC10.7751937984496124No Hit
GTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTT10.7751937984496124No Hit
AGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAATACAAAGACTGAAT10.7751937984496124No Hit
CCCCTGGTGAATGGCGGGGTGTTCCTCCAGTCCCTGCTCTGGCGTTCCGC10.7751937984496124No Hit
GTTTTGGGCGGGACTTTATACTGCAGTGGAAGTGATGCTGAGACCACGGG10.7751937984496124No Hit
ACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAG10.7751937984496124No Hit
ATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCA10.7751937984496124No Hit
TTGTAATTCTTTTGCACCTTTTGGGGGGTGCTTTTATTACTTTGTATCAG10.7751937984496124No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT10.7751937984496124No Hit
GTGGAAGTGCATGGATTTTGGGAGGAATGGATATTGGAAAAAGTGGTAAA10.7751937984496124No Hit
GTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGT10.7751937984496124No Hit
AGTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGA10.7751937984496124No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA10.7751937984496124No Hit
GGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGG10.7751937984496124No Hit
ACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTG10.7751937984496124No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT10.7751937984496124No Hit
TTCTATGACTAAAAAGTGATGAAATGCAACTAACCAGCTAGTCAGCTTTG10.7751937984496124No Hit
GTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAAT10.7751937984496124No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATACATTT10.7751937984496124No Hit
GATTAGTTCAACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTT10.7751937984496124No Hit
GGGGTTGGTGATGGGGGGGGGGGCGGGGTGGCGGGGGGGGGGGGGGGGGT10.7751937984496124No Hit
TGTTGATTCCCACTAACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCT10.7751937984496124No Hit
AATCTTCATTCTTTTACTGTCTAAGATGTACCCTTTCGGGTGTCTTGCCT10.7751937984496124No Hit
GGGCGGGGGGTGGGGGGGGGGGGGGAGGGGGGAGGGGGTGAGGGGCGGGG10.7751937984496124No Hit
GCCCGAGCCACCATGGCCTCCACCCTGCGAGCAAGTACCACCGGGCGCCG10.7751937984496124No Hit
TGAGGATCCACATGAACAAAATTCAAAAATCCAAAACCATCCCATAATAA10.7751937984496124No Hit
GGGCAAACCAGCGACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAA10.7751937984496124No Hit
GGCCAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT10.7751937984496124No Hit
GTGGGTATGCTGGCTGCACGCCCAGCGATGTGTGATTCTGTCTCTCTTCT10.7751937984496124No Hit
TTCCCTAGGCATCCCATTTTGTATTAGAACATAATAGGTCCCATGCTCAA10.7751937984496124No Hit
GTGGGTGGGGGGGGGGGGGGGGGGTGGGGGGGCGGGGGGGGGGGGTGGGG10.7751937984496124No Hit
GATCTGGCCTGCGGATGCCTTTTGTTGATTGGTATTTCCTCCACTCCTGG10.7751937984496124No Hit
CATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCA10.7751937984496124No Hit
ACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGA10.7751937984496124No Hit
CTCAAAGAGCTCTGCAGAAACCAAGATTTTTCTAGAATGTGACTCTTCCC10.7751937984496124No Hit
ATCGGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC10.7751937984496124No Hit
CTTTTACTCTCGAACATGTACCCTTTCCCTAGTCTTGCCATTTTGTTTGA10.7751937984496124No Hit
AATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAG10.7751937984496124No Hit
GTACTGGGACTAGTGAGGTTGAGTGGTAGGGTTCTGTACGCGTGTTGTGT10.7751937984496124No Hit
TTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATG10.7751937984496124No Hit
TAACCGGCTCTATCGTGTTAACTCAGATCCCGATATATGTCAGATTATCT10.7751937984496124No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA10.7751937984496124No Hit
ATATTGCACATATTGATGATTCACATCATCGGTCTCACAGACAGATCCCA10.7751937984496124No Hit
TTATTCGGCATGATGGATGGGGGGGCTCCGTGGCCGGGAGTGGGTGGGAG10.7751937984496124No Hit
GGTTTAGATGGATATAGAGATCTTAGACGCGATAAGAGAAAAGGAGTTAA10.7751937984496124No Hit
AAAGAAAGAAATGTCTACATACGTCTTCGGGTAACGTCAACCTCCAGCAA10.7751937984496124No Hit
GTGGCAGTAACCAAGTTTGGATTCAACGACCTCCTTGGGGATATCGGATA10.7751937984496124No Hit
CTTGAGGACGACACCACCCCCCCCCCGCTTGGGGGGGGGGCCACAGGGCC10.7751937984496124No Hit
AGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCG10.7751937984496124No Hit
AAACAACCCACAACAACACGCTGCACAGCCGCTACGCGGTCCAAAAGGAC10.7751937984496124No Hit
GCCCCCAATAGTGCTGAAATTTGCTTAACCGAGGTCCGGTAGTCCCAGGG10.7751937984496124No Hit
TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAA10.7751937984496124No Hit
ATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAA10.7751937984496124No Hit
CTGTCTGGCTGNNANNNNNNNTNNNNNNNNNNNNNNNNNNGNNNNNNNNN10.7751937984496124No Hit
GTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTT10.7751937984496124No Hit
GGTCGGAGGGGCGCAGGTGCACATAGGTCCTGCCGGCCGGCTTGTGTGTA10.7751937984496124No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAC10.7751937984496124No Hit
GGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAC10.7751937984496124No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG10.7751937984496124No Hit
GGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCT10.7751937984496124No Hit
CAAATGATCTTCTCGTCTGAATTTGTGTGTCTCCCCTATGGCACCTATAT10.7751937984496124No Hit
GACACACATTCACATCTTTTCATTCACTGGAGAGGAGATGGCCACCAAAG10.7751937984496124No Hit
CCCCTGGGGGCCCCGGTGTTACCCTTTTCCTCACCTCCGGAAGTCTACTG10.7751937984496124No Hit
CAAAATGACCATCGTCAACATCCACAGCACTCTGCTGTTCCTGTTGATAT10.7751937984496124No Hit
GGCCGTAAGGATGGTCGGGGGCGGTGGGGGGGTGGGGGGGCGCAGGGCGG10.7751937984496124No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT10.7751937984496124No Hit
TTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGA10.7751937984496124No Hit
GGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCA10.7751937984496124No Hit
GATGGGGCGTGCGGGCGGGGGTGGGGGTATGGGCTTGCGGTGGCGGGGGG10.7751937984496124No Hit
TGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTA10.7751937984496124No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG10.7751937984496124No Hit
AAAATGGAGGGAGTGCCTGCGCCCACGTCCTCCACATCGCACCGGTAGCT10.7751937984496124No Hit
GTTCAAGCATTTTGGTTATTGGGATTGGTGGTACTATGGAAACAGGTGGA10.7751937984496124No Hit
GGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGA10.7751937984496124No Hit
GACTACCACTGTGGTCGCTTAGATAGCCATTCACCCGTACTAGCGTGCAT10.7751937984496124No Hit
CTTTAACAACAGAAGATGCCTGGGCCGAGGCTTGGGTGTTTGCATATCCT10.7751937984496124No Hit
TGCTTGAAAGAGAATTGGTCGTTAAATCAAGGTTTCTCCAAGGAGCCGGC10.7751937984496124No Hit
GATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTATCAAT10.7751937984496124No Hit
GTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGT10.7751937984496124No Hit
GGCGGGGGCCGGGCAGGTTGGGGCGGGGGGGGGGGGTGGGGCGGCGGGTG10.7751937984496124No Hit
TTTCATAAGAGGAAAGAAAGTGATTCCAAGAGGAAAGCTTTCCACAAGAG10.7751937984496124No Hit
GTCCAAAGTCAGCATCATCCAGAGACTGGGCAAGGCTGCCCTTGTGCCCG10.7751937984496124No Hit
CTCTCACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT10.7751937984496124No Hit
GAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGACCAAGG10.7751937984496124No Hit
CATCACACACAAAAGGGGTAACTATGGGAGCTGATGGATGTATTAATGAG10.7751937984496124No Hit
GTGTGTGTGTGTGTATACTTCTGTACTTATATAGTTAAAACAGTATTAAA10.7751937984496124No Hit
GGTGGGGGCGGGGGAGGTGGGGCGGGGGTGAGGGGCGGGGGGCGGGGTGC10.7751937984496124No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.7751937984496124No Hit
GGTGGCACCGGATGGGGGTTTGGTAGATGGGTGCGCATCGGAGGTGGGGG10.7751937984496124No Hit
GACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG10.7751937984496124No Hit
CGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTTACAAA10.7751937984496124No Hit
GGTTGTCAATGTGTCTTCCCGGGAGAGGGCTTGGTACGAGGCGATGGAGT10.7751937984496124No Hit
CAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACT10.7751937984496124No Hit
ATTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT10.7751937984496124No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers