FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTAAAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCA14.3478260869565215No Hit
TCCTTTGGTTCCTTGACCCCAGTGGGCCTCACTCCTGCCACCTAGTTCCC14.3478260869565215No Hit
CCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGG14.3478260869565215No Hit
GTCTGCTATGTCACCTGCTCTTCAGTCTTTGTGTCGAACAGAGTGATTCA14.3478260869565215No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA14.3478260869565215No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG14.3478260869565215No Hit
GTGCTCATATTAGATATCCACTCTTATTCAGACTTAGTATTTTCTTCCCT14.3478260869565215No Hit
ATTAAAAAGTGCAACCCATAATTATAATGTCTCAAATTAGAACTTCATTT14.3478260869565215No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTTAAG14.3478260869565215No Hit
GTAAAGTTCATTACCATAAGGTATATAAAACTTATTTCGAAAAGGTCGAA14.3478260869565215No Hit
ATAGCAGGCAGTGTTCTGGGCCACAGATAGAGGAGCAATATGCAACAGGA14.3478260869565215No Hit
GTAGGGAACTCTACGAGACCGAACTGTGACAAACAGCCTATCAGGACGAC14.3478260869565215No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACAT14.3478260869565215No Hit
GATTAATCCATGGCCAGAGCTGCCATGAAGCCCGGGGGCGGGGGCTTATG14.3478260869565215No Hit
AATTAGCTCTATTCTAGACAAACCGCTGAATTAAGAACAAGATTAACCAA14.3478260869565215No Hit
GGCTTATTCTGGCAAAGAGACTTTTACATTCTAGCCCAAGCAAGCAATCT14.3478260869565215No Hit
ATTTAGGAGCGCTAGGACCCCACACTAGCTTTCATCACTGGATGTGGAAC14.3478260869565215No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA14.3478260869565215No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT14.3478260869565215No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC14.3478260869565215No Hit
GATTCCAGTGTGGAAGAAAGAATCCAGCTCCTGAGGTCCTCCTGTTATGC14.3478260869565215No Hit
CATAGAGGTCAAAGTAAGATGTAGCCACAAGCAGTCATTTACACAGACTA14.3478260869565215No Hit
GGTTAAGGGTCTGCCTTCAGCTCAGGCCATGATCTCAGAGTCCTCAGATT14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)