FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACTACACCTCGGGAGTAGGGTCGTCTTTAATTTATAAGAGAGCAAG15.0No Hit
GCCACAAAAAGAGCAATCACTCAGTGCAGAGACCAAACATTCTACTCAAA15.0No Hit
ATCGGGGTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTACCCGCCCCTG15.0No Hit
ACGGTGTTCATGTGGTGCACTGGCCAGATGCATGGGCCTCAAAGACACCG15.0No Hit
CTTCATACCCTTAATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT15.0No Hit
CTACGTCCCCTTGCCAAATTAGAACCTTAGCCTTATCTCCTTTTGCAAGA15.0No Hit
GGTACAGACCGAATCATGCCCTGGAGAGTGGTCAACACTATTCCATTGCG15.0No Hit
ATTCAGTATCGATCCTCACAAGACATGTCAAACCATCTTGAACATGACAA15.0No Hit
CTCTGCGGGGGCTGGTGGAATTCCAAGACGTCTCTTTTGCGTATCCCAAC15.0No Hit
TTGCTCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15.0No Hit
TTATACAACAAAAGGGAATTTGAACGAAATCATTCTATTGTCCATATGGG15.0No Hit
CCCCGGGGTGACTCTCCGAGTGGCCATGGTGCCGATTTGTTTTCAAAGAA15.0No Hit
TTAGAAGAGGGCTTATTTTCTCATTGTTGGTGTGTGTTCAGTAATAGAAT15.0No Hit
GGCCTCTCCTGCTTGTATTCCCTCATGGTTGGGTGCAGTCCGTTTGAGCG15.0No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15.0No Hit
TCAGTATGGATTCAGGAGCCCGGCAGTGGGCCCCGTCGGCTGTAAAAGAG15.0No Hit
ATTCTCTTTCTAGCATGTACGCCACCATCAAGGGAGCAATTTTAAAATCC15.0No Hit
CAAGCCGGTTCGACCATCAGGGTAACTCGGTTAGCAAATACTACGATATA15.0No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG15.0No Hit
AAACTAATTGATGGCCATCCGAATTCTTTTTGTCGCTGTCTGGCTGTCAG15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)