FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA70.38022813688212925No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT60.32590983161325365No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC40.21727322107550243No Hit
GTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAGGTCCA40.21727322107550243No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC30.16295491580662683No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATACTGGGAAGGGAGACACG30.16295491580662683No Hit
GCTATTATTAATTCTTTAATGATAGATACAACCACCAAAGCAAACTGAGC20.10863661053775121No Hit
GGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAAGCCTGATGTGGGAC20.10863661053775121No Hit
CTCAGGGATGTGTAGCTAGAGGAGATCTGGCCCTTGGCTTCCAAGTACTC20.10863661053775121No Hit
ACACCGTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGGGTGATACT20.10863661053775121No Hit
GGGGGTGATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGG20.10863661053775121No Hit
GGCTGGACCTGTCCGGGTCCCCTGGGGAGAGGCAGTAGGCAGGGGTTTTA20.10863661053775121No Hit
TCTTACATGAGTCCAACCGTTCAAAGGGTAATGACTGCTCAATTTTTAGT20.10863661053775121No Hit
GGATGTACCCAGCCCAGAAGAGTAGCGGCCATGTCAGTGGCCAAAAGAGT20.10863661053775121No Hit
CCGTAGAAGCATATCCAGTTTGCAGGAATGTAAATCTAACGAATTCTCAC20.10863661053775121No Hit
CTTAACCCACTGAACCACCCAGGCACCCTGAAAACTCATTCTCTATTGGC20.10863661053775121No Hit
CTTCTGAGAGGAACAAAGGGAAAACATCATTTCCTTGACATTTCTTGTTG20.10863661053775121No Hit
GTACAGGAATCAAGGCCTCAGTAGCTGGGCTGATTTGCCCAAGTGGTGCT20.10863661053775121No Hit
GGGTGATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGG20.10863661053775121No Hit
GAGCAAGAGGCAGGCCTGCCCCATGGGACAAAGCTTCTAGAGCCAGGTGG20.10863661053775121No Hit
CAGCTGGGCTGCCTGCCTGCTGTTCATCTGTTGCGGCACCTCCCCTAAGC20.10863661053775121No Hit
GGTCTAGGCAGAGGCGCTTGCCAGTAAGGTACGCAACAGCATCAACAAAA20.10863661053775121No Hit
GCCCAGATCCTTGGCCCTTATCCCTCCTTTGGTTCCTTGACCCCAGTGGG20.10863661053775121No Hit
AGCTATTACTGCAAAGCCCGAGAGTTGGGCTTAAATTTGAATGAGGTTGT20.10863661053775121No Hit
CATAGAGACAGAGAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTG20.10863661053775121No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG20.10863661053775121No Hit
GGGTAGTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAA20.10863661053775121No Hit
CCCCCTAAGATCGGCCACCTGAACTCCAAGCTCTTCCTGAATGAGGTGGC20.10863661053775121No Hit
AAATAGATGTATTATGCCCCAATACTCAGCAGCAGGGAAATGAGGATCTT20.10863661053775121No Hit
CCTCTCCCCCAAAGACCAGAAAATTGCTCCAGGTCCAACTGCCCAGATCC20.10863661053775121No Hit
GGGTAGTTGGGAAAGAGGCTGATATCCTCCAAGAAGATACCTACAAAATG20.10863661053775121No Hit
GGTCATACTGCTGGGGCCCCAGCCAGGACTGGCTAAATGTCTACAGTGGG20.10863661053775121No Hit
CCTTCATTTTACTTGATTTATTAAGGACTGTGACTTGTGAGTCAAGAAGA20.10863661053775121No Hit
TCCTGGGATCTGGCAATTCTTAGTAGTTTCTACCCTTTTTTTTTTTTAAT20.10863661053775121No Hit
CCTCAGGACGCCTCCATTGTCCCACACGCCTGTGTCCTCGAACACTGCGG20.10863661053775121No Hit
GTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATT20.10863661053775121No Hit
CTGTAGGACTGAGCACATCTCCTGTGTTGCCCCAGGGATGTGTTTCTGCT20.10863661053775121No Hit
GCCTCTCCCGTGTTTGTGAGGAGAGGCAAAAGCAGAAGTCCAAAGAACAT20.10863661053775121No Hit
GGATTTCCCATGACCCCACTCAGGACCCTGAGCGGCTCCTGTCCCAAGGC20.10863661053775121No Hit
ATCAAGGAAGAAACCAGAACCCCTCTAAATGTCCCTTGCAGCGTGGAAAG20.10863661053775121No Hit
TTACCCACCCCTTTTGGCAAGGTGAGGGCACAGAGAGTGGGAGAAGTGTG20.10863661053775121No Hit
GTCTATAGAAGAGACCAAAGTGGTAGGCTGTGCCTGTGCCACTGGCACTG20.10863661053775121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACC100.00651747145.01
TTAACCC100.00651747145.02
Produced by FastQC (version 0.11.5)