FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG170.9626274065685164No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG120.6795016987542469No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT70.39637599093997733No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG70.39637599093997733No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG60.33975084937712347No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG60.33975084937712347No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA60.33975084937712347No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC60.33975084937712347No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT50.28312570781426954No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG50.28312570781426954No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA50.28312570781426954No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC50.28312570781426954No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA50.28312570781426954No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC50.28312570781426954No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA40.22650056625141565No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG40.22650056625141565No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG40.22650056625141565No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC40.22650056625141565No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA40.22650056625141565No Hit
GAATTCCTTGGTCATGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCA40.22650056625141565No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT40.22650056625141565No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA30.16987542468856173No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG30.16987542468856173No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT30.16987542468856173No Hit
ATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCT30.16987542468856173No Hit
GCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTC30.16987542468856173No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT30.16987542468856173No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT30.16987542468856173No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT30.16987542468856173No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.16987542468856173No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG30.16987542468856173No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.16987542468856173No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.16987542468856173No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC30.16987542468856173No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG30.16987542468856173No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA30.16987542468856173No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT30.16987542468856173No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA30.16987542468856173No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC30.16987542468856173No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.16987542468856173No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.16987542468856173No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG20.11325028312570783No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA20.11325028312570783No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT20.11325028312570783No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT20.11325028312570783No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC20.11325028312570783No Hit
CTGGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGC20.11325028312570783No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT20.11325028312570783No Hit
ATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTA20.11325028312570783No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT20.11325028312570783No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC20.11325028312570783No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA20.11325028312570783No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA20.11325028312570783No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAG20.11325028312570783No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20.11325028312570783No Hit
TCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGC20.11325028312570783No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA20.11325028312570783No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC20.11325028312570783No Hit
GTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTTGGCATTGTTTTTTA20.11325028312570783No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA20.11325028312570783No Hit
GATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAGAATCTGG20.11325028312570783No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA20.11325028312570783No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA20.11325028312570783No Hit
ATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGAT20.11325028312570783No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC20.11325028312570783No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG20.11325028312570783No Hit
GGCTAACATTACCATACAGATGACTGTGCTTTTTAAAGTAACAGACTCTG20.11325028312570783No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC20.11325028312570783No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT20.11325028312570783No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT20.11325028312570783No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT20.11325028312570783No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC20.11325028312570783No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.11325028312570783No Hit
TCTATAAGCTTTGCTCCAGAAACGAAAGCAGCAGGCACCTCCTCCCCTAT20.11325028312570783No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT20.11325028312570783No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA20.11325028312570783No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG20.11325028312570783No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA20.11325028312570783No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA20.11325028312570783No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.11325028312570783No Hit
CATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT20.11325028312570783No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT20.11325028312570783No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGACGAGCTTGC20.11325028312570783No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC20.11325028312570783No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20.11325028312570783No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.11325028312570783No Hit
GCCTAGTTTTGATTCTTGCCCTGCTCTCTTCGTCAAGGGTGTAGTCCGCT20.11325028312570783No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA20.11325028312570783No Hit
CAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAG20.11325028312570783No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG20.11325028312570783No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA20.11325028312570783No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT20.11325028312570783No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC20.11325028312570783No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA20.11325028312570783No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA20.11325028312570783No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.11325028312570783No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA20.11325028312570783No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG20.11325028312570783No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC20.11325028312570783No Hit
AAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAAT20.11325028312570783No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA20.11325028312570783No Hit
ATTTAAATAAATAAATAAATGCACCAGTAGAGAAGGCTATGTATAATAAG20.11325028312570783No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC20.11325028312570783No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGACACAGTAAAC20.11325028312570783No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT20.11325028312570783No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20.11325028312570783No Hit
TCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGC20.11325028312570783No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG20.11325028312570783No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)