FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTTCTGCAGATCCGAATGATTATGAACTGTAGGAGGATAGAAGGTAGT13.125No Hit
CCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAAT13.125No Hit
TATTTAAACAATTAGACACTAATTGATGGCCATCCGAATTCTTATCGAAT13.125No Hit
AAAATATTGAAGACAAAAAATAGACAATTTATATATTAAATCTCTAAGGA13.125No Hit
ATCTAAGAGATCTGATCTATCTGGATTCGTCCTGACTCGAAGTCTGACAT13.125No Hit
GACTGTGTGTCTGGCACTGGCCTAGGCACCTTTGCTCACTTAAAAATACC13.125No Hit
CTCGGATTCAGTCTTTGTTTTTTATTCCCTATTGTTCTTTTCGTGAACAT13.125No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG13.125No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT13.125No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT13.125No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT13.125No Hit
TTGTTGATCCGACAAGACTCGCAGTAGCCCCAGGCAAACAAACTCGCCCT13.125No Hit
GAGCATGATGACAGAGGATGAAATGTCAAGAGTTTGCAGACCAGCACAGT13.125No Hit
GAATAAACAACCAAGCACAAATGGTACAGTGAACAGGAAAGCACTAACAT13.125No Hit
ATCCCACAGCTTCTTTAACTCAAATGATATTCTCGTCTGAATTTGTGTGT13.125No Hit
TCTTAATACTTAGCTGATTACAATAGCGCTGTGTGGGGAGCAAAAGAGGA13.125No Hit
ATCTCAGAGAACTCTTATATATTGATCCGTACAGACTCGAACTCTAAACA13.125No Hit
CTCTGTATGGTAGGCGATGTGCATTTTTGTCTTGTCTTCCAGGTCAATAT13.125No Hit
GCAGTACCCTAAGGTAGATAAACCGTATGTCGAAAAGTTCGAAAGGGGGC13.125No Hit
ATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTC13.125No Hit
ATTCATTTTGGAGAGAATAAAAGAAAGGAGAGATCAAATTTATCAGTAAA13.125No Hit
CCATCCATCTACTAGTGCTGCCCAACAAAGTCTCTATAAGAATGCAGATG13.125No Hit
ATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTTTGTCAGT13.125No Hit
AAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGG13.125No Hit
GATAAGTTCAGCTTTAATTTGCTCTGTTTCTAGTGTCTTGTGGAGGAGCC13.125No Hit
TCTCCGCCCACTTGTGATCTCTTGCTGTCAAATAAATAAATAAAGATAAA13.125No Hit
CAAACGCTGCGTGATGAACGTCCCCAGCCTGTGCGGCACAGACCACACGG13.125No Hit
GTCAATGGGGTGTGTGACCCCGTTCTCCTTCAGCACCTCGTGTTTGCTGG13.125No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC13.125No Hit
CGTTACATACGATGACAGGTGAAGGAGTATGTTTTAAACATACATGAATA13.125No Hit
TCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGAC13.125No Hit
AGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACTGTACAG13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)