FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAAGCAGGAACGCTGAATCATATAATGACTAACAATGACGACTAAT10.9345794392523363No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATACTGGGAAGGGAGACACG10.9345794392523363No Hit
CTATAGGGCATAATAGTAGGGAGAGCACCAGGCATTAGAGGTCATAAGGC10.9345794392523363No Hit
TTTCCATGGTTGCTGGTGTTGATGGCACGATCTCTGTTTGGGCTAAGGAG10.9345794392523363No Hit
GTATAAAAACCAACAGGTGGTGACTTACGAAGGGAAACGGGAGACTGGAG10.9345794392523363No Hit
ACTTAATAATATCCTCAAGGTTCATCTGTGCTGAAGCACGTGACAGGCTC10.9345794392523363No Hit
GGACACAAGCGTTCTAATCTCCCCTGGTTCTTGGCTTCTTCATTTGTACC10.9345794392523363No Hit
TTTTTAAACAATTGTACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.9345794392523363No Hit
CAGCATAAGACAATGTCTACCACTCCTAGGAACTAAAGACACAGATTCTA10.9345794392523363No Hit
CTCACATAGGTTGCACGCACGCTCTCTCTCTCTCTCAAATAAATAAATAA10.9345794392523363No Hit
GAAGACCCGAGTCACGAGGGGGAGGGAATACCACTATATGATGCAATCAA10.9345794392523363No Hit
TCATTTTGACAGAGTCAGGTATTTGAGGAATGATGAGGTGTTTTTTTTTT10.9345794392523363No Hit
GAATTAATAGATAATGTAACACAAAGGTGGTAGTGCATGGTCCCTATCCC10.9345794392523363No Hit
CTCACGCTCTGACCGCTTAAGAATTTTGGATGTGTGCAATCAGAAAGTCT10.9345794392523363No Hit
GATCCTAGCTCCAGTGCTGGGCTGAATGATGACCTTCGTGAAAATTTGCA10.9345794392523363No Hit
TGGTGGGGGTGATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTT10.9345794392523363No Hit
GCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGG10.9345794392523363No Hit
GAGCCACAGATGGCCAAGAGCAGCCCCCCAGAGCAGCACCTGAGCTGGCC10.9345794392523363No Hit
GATGGTGCCTGCCCTCACGCCTCCCCGTGAAGAGTTGCTGCACAGCCGGA10.9345794392523363No Hit
ACAGGAATCAAGGCCTCAGTAGCTGGGCTGATTTGCCCAAGTGGTGCTCG10.9345794392523363No Hit
GTCCATTTTTGCCCAAGTGATGTGAGATGCAACCTTTATCATATATTAAA10.9345794392523363No Hit
GTATTTTGATAGGTATTACATCAAATATGTAGATTTTGGGTAGTATAGAC10.9345794392523363No Hit
GCTGGTAAAGGTTCTAGGACCCCAGGGAAGTGTGGCTTCAGTAATGAGGG10.9345794392523363No Hit
TGCCAACTGTGGCATACCTGTGAGAGCATAAACCGAGAAAAAGCCACTTT10.9345794392523363No Hit
GCGGAGAGCCTGCTTCCCCCTCTCTCTCTACCTGCCTCTCCGCCTACTTG10.9345794392523363No Hit
CTTGGGCTCTGGCTTGCCCGTTAAAAAACCCCAGAGAAAACAGTTCCTGC10.9345794392523363No Hit
GTTAATACTTCCCTTAATTACCCTTTATCTTTTGAGTTTCCTGCTCTGTA10.9345794392523363No Hit
GGATTTCTACGTGAATAGCCCTAAGTGGCTCAGCAATAAATTTAGTGTTA10.9345794392523363No Hit
CAGAAGTAGGAATGCCCTGAGTGGTACTACCCACATGCATCCATGTGTGA10.9345794392523363No Hit
GTGTGACAGGAGTGTGGCTGAGACAGGGAGGGAGAGGGGGACATCATTCA10.9345794392523363No Hit
TCACTGAGTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGT10.9345794392523363No Hit
ATTCAAAGGACTACATATCATTTAATGTTCCAAGAAACCGAACAATAACA10.9345794392523363No Hit
CAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATT10.9345794392523363No Hit
CCTCTCGAGTCTGCAGGTTCACTTCCACCACTCATTTTATTTGGAAGTCG10.9345794392523363No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA10.9345794392523363No Hit
ATCGTATACGTGGGGGAACTACGTAGGAATACAGGACTAAAATCTCTAAT10.9345794392523363No Hit
CACATGAAGTAAAGTTCTCGGCTGAACAAATTGTAAGCAAATATTATGGG10.9345794392523363No Hit
GCGGGCTGGAGAGAAGCACAAAGGGTTTCATATTGCCATCGGGTGGGGGG10.9345794392523363No Hit
GTCATAAGGAAGAACACCTAGAAAGTCAGGTACTTAAAAATACTGCTGGA10.9345794392523363No Hit
CTTCTTAATAATAAAGGATGCAAAATATATATATATGGGTGATAAAAAGA10.9345794392523363No Hit
GAGTTCGGTTTTGAGGCAGGGTCGGTGAGTGGTGTGGTTTGGGGGGTGGG10.9345794392523363No Hit
GTTTGCACGTGATATTTTCTTTCAACATCTCTGAATGAATTGATGGGTAG10.9345794392523363No Hit
GGTGGTAGGCGCCCATCGTGCAGGCACTGTCAAAGAACTTCTGGTAGTAG10.9345794392523363No Hit
GTCTGGGGACGTGGGAAATGGAGCCAGGAGAGCCACTATCCCAAAGAGGG10.9345794392523363No Hit
ATCTGTGAGAAGGCAGCCAAATGATTGCAGTTCTCTGTGGCCTCTGATAG10.9345794392523363No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC10.9345794392523363No Hit
ACCGCATACTTACAATATAAGAAAATTTTAAGGATGGCTACTTTATCATA10.9345794392523363No Hit
GTTTTGGGGGGCGTTCGGGCGTGTGAGGAGTGGGGGTGTTGGGTGGGTTT10.9345794392523363No Hit
CTACATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGCTTGTGA10.9345794392523363No Hit
GCTCGGGCCTCTGGGTCATGACTGTCATACTGTTTAAGGTCTCCAACATC10.9345794392523363No Hit
GAAGGGAGGCGAGCATCTGGATTCTAAACGACTTGAGTGTGGGAAGGCCA10.9345794392523363No Hit
GGGGGAAGGAGTTCCCAGAGCAGGATGGCAGAGCCAGAAAGCCAAAGTGC10.9345794392523363No Hit
ACTGTGGGTATCGTCACCCATATCAATACTGATTGTCATTAATGGACCCT10.9345794392523363No Hit
GTGGACGTTGGTGGGCACATAAAATGATACAGCCAGGAAGGAAAACAGTA10.9345794392523363No Hit
GAAGACATTCTCAGCATCCTTGCTGAAGTGAATTTTGAAGTGAGCAGTAA10.9345794392523363No Hit
CCCGAGAGGGAGGTGAGTGAGGGGCTGGCCAGTGGCTGTGGTGAGCAGAG10.9345794392523363No Hit
GGTCTGGCAGGCTGGGAGAATGCTGGCGGTACGGAAAGGCTGGCGCTGCC10.9345794392523363No Hit
GCTATCACAGAGAAAAGTACAGCAAGGTAATCCTAAGAGTTTGGGAAAAG10.9345794392523363No Hit
GATTAAGAGTCTCTTATGTTTTGTCTCCCTCACGATCCCATCTTGTTTCA10.9345794392523363No Hit
CCGCTGAGCAGGGATCCTGATGTGGGGTCAATCACAGGACCCTGAGATCT10.9345794392523363No Hit
GGGGAAAGGCTGGCCTGTAGGGAACAAGGGAGGGACAAGAAAGCCATGGT10.9345794392523363No Hit
GTCTTCGATGTCATAGGAGATGGACTCGCTGGTGCTCTCTGACTCCCAGG10.9345794392523363No Hit
CAATTCAACTTGGGAATCAAAATCAGATTGAAACATGCAATCAAAGCGTC10.9345794392523363No Hit
ACATCATTCTGGCTAACATTTATCTCGAAGAACAGATTAAGCCACAGGTA10.9345794392523363No Hit
GTGTGTACCCGATCGGATCCCGTATGTTTAAACATGTAGAGTGTTGTGTC10.9345794392523363No Hit
CATTAATTGCGAGGAAAAGAAATGTTCATGACCTCAAAGAACCTATCTCC10.9345794392523363No Hit
GAGTATGAAAACTTCAAGCACCTCCTGCAGGCACCGCTGGATGATGCCCA10.9345794392523363No Hit
AATCTTCATTCATTTACTCTCGAACAAGTACCCATTCCCTAGTCTTGCCA10.9345794392523363No Hit
CTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTG10.9345794392523363No Hit
ATCAAAACCAGAATAATTAAGAATAAGTAGCCACTCTGTTCACCAGACTT10.9345794392523363No Hit
CTAATTGTTTGTGAATGTTTTCAGAGGTAGCACGTAATGAACCAAGAGAA10.9345794392523363No Hit
GGCTTGTCTGTCCTGCCACCGCCGGCGACGATGCCGACCATACCGCGGAC10.9345794392523363No Hit
TGTCGATACTGTGTATGTTAAGGTCTGTGACAGACACAAAGCTTTTCAGA10.9345794392523363No Hit
GGGGAGGGCCAAGCGCACAGAGGACAGCTGGTTGCAGTGTGTCCAGAGAG10.9345794392523363No Hit
GTCTAAATACCGTTCAATACATATTTGATTATTCAGAGCTCCCCCAACTT10.9345794392523363No Hit
CCCTGAGAACAGTGAGAAGCACAAGAAGGGTATTAAGCCATCTCAGGATG10.9345794392523363No Hit
ACTTTGGGCAGGGTGACCTCCTTAAGTCTCATTTCTCTCAAGGACAAAGT10.9345794392523363No Hit
GGAGCGAAAGCAGGGAAAGGGAAAAGCAGGCTCACCAAGCAGAGAGCCCA10.9345794392523363No Hit
AAGTATGTGTCTCCTGCTTGAATTGAAACATAAAACAGGATCAACCTCAC10.9345794392523363No Hit
GGGGATTACCAAGTCTTCCCCTATAATCATCATCCATTAGCTCCCAGTTT10.9345794392523363No Hit
GAGGGGACAGGATTGTAGGTGTCTTCAGAGTTCAAGTTCGCTCTAAATGG10.9345794392523363No Hit
GACTGGAACCACCCCTGTTCCAGTGGTGTCACACCCATGAGCCTGTTCCT10.9345794392523363No Hit
ATGGCAGCCAGTCCCTGGGCCCTGACAGGAATCCCAAGAGGGTGTGGTTT10.9345794392523363No Hit
GCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGAT10.9345794392523363No Hit
GGCTCGGTCCCGGGGCCCGGAGGGGGTGGGCGGCGCCGGGGGCGGGGGCG10.9345794392523363No Hit
ATCAAAGGTAGGGCTATTACTATCAGATGGAGGACCAAACTTATACAATA10.9345794392523363No Hit
GCTAAGGCCTGACGTGGGAGCGGGGGTCATGGGGCCCAACCCAGGAGAGA10.9345794392523363No Hit
CAGAAGCTAGTCAACCAACAATTGTCAATTATTCATAGCCTTCATGAACT10.9345794392523363No Hit
GCGCTGGAGGTACCAAAAGAGTTGGAGTCAAAAGGGCTTGATATGAGGGA10.9345794392523363No Hit
CATCAGTCGGAGGCAGGAGGTGGGCTGTGCTGGCGGCTGGATAAGATCAT10.9345794392523363No Hit
CTTTGTTACCGGCCTGTCACCAGCCAACACACAGACGGTTCTTCTAGGTG10.9345794392523363No Hit
GTGTTGACATTGATGACATAATTTTCTAAAACAGTGAATAACTCTGGTAA10.9345794392523363No Hit
GTCTTCTAACCGAGGTCGAAACGTACGGTCATTCTATCATCCCGTCAGGC10.9345794392523363No Hit
GAAGTATTTTAGGCATTCTGAGAAGAATCAAGTACTTTTCTTTCCTATCC10.9345794392523363No Hit
CGATAGCAGACACTGTTGAATGTTTCATATGCTCTAGGCACATTTACCCC10.9345794392523363No Hit
CCAGACTAGATTGACAAAAATTCAGAGCAGAACATCAAATGAGTTAAACA10.9345794392523363No Hit
ATACAAATACCCAGTTCAGAGTTTTAACTGTCTTCATTTCTCTCTCTCTC10.9345794392523363No Hit
CCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCAC10.9345794392523363No Hit
AATCAAAGGACATAGGGTATCAGGTTGGATTAAAAAAGGAAGAAAACAAG10.9345794392523363No Hit
CTGAATACCCATTTTCTCGGTATCAAGGTAGATCCCTGAGAAATTCGCCA10.9345794392523363No Hit
GTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGTTTCTGC10.9345794392523363No Hit
CTTTTTAGCTAAGTCGGGTCCCCAAAGTCTCCCTCAGTGCCCTCGGAGAG10.9345794392523363No Hit
CTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATAT10.9345794392523363No Hit
GAATAGAGAAGAATGCCAGGAGGAATGAGAAAGGATCAGCAAAACATTTA10.9345794392523363No Hit
TCTCTATACTGAGGATAATCTCGAGCGTGCCCTCTCCCACCCCGGCCACG10.9345794392523363No Hit
CAAGTATGTTCTGGATACGGGTGGGAGATGTGGATAGAATGCTTAACAGG10.9345794392523363No Hit
GGATTATATCTCAAAAAAGTGCCCTTTTCCAAAAAACAAGAAACAGAAAT10.9345794392523363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA400.001378013818.12590-94
Produced by FastQC (version 0.11.5)