FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_F03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_F03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGTCACCAATTCAATCATCTGCTATATAAATGAGCAATAAGAAGATT16.25No Hit
GTGTTTGTGCCGAGTTGGAAAAGGAGGGAGTTACCAGCTGGACAAACAGA16.25No Hit
ATCCAGATAGATACACTAAATTCCCCTGCGTTAGTGAAGTCATTTTGGAC16.25No Hit
GTTCTATAAATAGACTGAAGTGCTTGGGACATATTCTGCCTCAACAATAG16.25No Hit
TCCTCCATCTGATACTAATAGCCCTACCTTTGAATCTGTTTTATCCCAAA16.25No Hit
ACACTAATTGATGGCCATACGAATTCTTTTGGTCGCTGTCTGGCTGTCAG16.25No Hit
ATCAGCCACTCCTCGATTGCTTAATATAGACACCCAAGCTCGAAGGTTCC16.25No Hit
GCATTGCAACAAATACTTCAGTGAATTTGTGAGGAAGGAGTTGAAACAAG16.25No Hit
CAATTGAAGCAGCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC16.25No Hit
CTGGGAGCCCAGACTGGCCAAGCAGTTCGCAAATGCTCCGAGCTAAAGAT16.25No Hit
GTGAAGGTCCCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATA16.25No Hit
GGCAATCTGATGTGGCGTGGTGTCGTCCTAAAGAATGTGTCAATGTTGGC16.25No Hit
CAAGAATTCCCCTTTGGATTGTGTTGAGAATAGAACGATTCCTCTTGGGA16.25No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC16.25No Hit
CGAATAAGAAGATAAAAATGCAAAATATACAATCATTAATTAACGACAAT16.25No Hit
GTTCACTTGATCCGCACAGTAATTATGAATAATGTCTCAAATATCAATCA16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)