FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGAGGGAAGTCCACATATATTACCTAGAGACAGCCAACAAAAGAACA14.3478260869565215No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG14.3478260869565215No Hit
GGGCGGGGGGGGGGGCTGAGTGGAGGGATGGGCGCGGGGGTGGCGGGTTG14.3478260869565215No Hit
CCATTTGGTAAGTGAAACAATACAATATAAACACTGACTGTGCCGCCTTC14.3478260869565215No Hit
GTAGAGACCCAGTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT14.3478260869565215No Hit
GGATCAAGGTAGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGA14.3478260869565215No Hit
AACTTACTGATTGATTAATGCTCCAGTAATTGTGCCAATTCGTCGTTAAA14.3478260869565215No Hit
ATATGACCTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGT14.3478260869565215No Hit
CCATTAAAAAAAGGTCAATACACACAACCACTCTCACCGAGCTGGAGTAA14.3478260869565215No Hit
AGCGAGGATGGTGCTTTCTGCGTTTGATGAGAGAAGAAATAAATAACTAG14.3478260869565215No Hit
CACATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGA14.3478260869565215No Hit
GGCAACCAGAAGCCGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTC14.3478260869565215No Hit
TGCCACAAGAGGGGTCCAGAGTGCTGCAAAGGAGAGTGGGGAACCCATGG14.3478260869565215No Hit
CTCCTTCCGTAGACGGCCCTCTGTTCACACGGTATTTAAAGCGACGAGAT14.3478260869565215No Hit
GGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGCATGCA14.3478260869565215No Hit
GCCTAAAACCCCTCAAGTTGTGGAGACGAAAATAGACTGTTGAATACAGA14.3478260869565215No Hit
CAATAAAGATGATTGAGGTCTAATGGTTCCGCATCTTGCCTATTCTAGAT14.3478260869565215No Hit
GGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATT14.3478260869565215No Hit
GTACCTCACTAAAGGGTTAAGTGGCCAGAACTAGGAGAGAAAACCCGGAA14.3478260869565215No Hit
TATATACCAATGTTTCTTGAACAACTTCCATTGTTTCAAGGCAAGAATTC14.3478260869565215No Hit
TGGTGATAACCACGAACTTGCAGGTCCTGGTGCCTCTGGCCAGTCCTGCT14.3478260869565215No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA14.3478260869565215No Hit
CTTTTGGCTGTACCATGGGCTGGCATTACAACAGCATTGTTTACCGAATC14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)