FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG150.6114961271911945No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG140.5707297187117816No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA120.48919690175295555No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC120.48919690175295555No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA100.40766408479412963No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA90.36689767631471665No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG90.36689767631471665No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT80.3261312678353037No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA80.3261312678353037No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC80.3261312678353037No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC80.3261312678353037No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG70.2853648593558908No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC70.2853648593558908No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC70.2853648593558908No Hit
GCATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA70.2853648593558908No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG70.2853648593558908No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT60.24459845087647777No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT60.24459845087647777No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG60.24459845087647777No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC60.24459845087647777No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG60.24459845087647777No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC60.24459845087647777No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA60.24459845087647777No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT50.20383204239706482No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA50.20383204239706482No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG50.20383204239706482No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG50.20383204239706482No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG50.20383204239706482No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG50.20383204239706482No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC50.20383204239706482No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT50.20383204239706482No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC50.20383204239706482No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA40.16306563391765186No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA40.16306563391765186No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT40.16306563391765186No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT40.16306563391765186No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA40.16306563391765186No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC40.16306563391765186No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA40.16306563391765186No Hit
CTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCATTGA40.16306563391765186No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG40.16306563391765186No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT40.16306563391765186No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA40.16306563391765186No Hit
GAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATC40.16306563391765186No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC40.16306563391765186No Hit
GAGATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTG40.16306563391765186No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA40.16306563391765186No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA40.16306563391765186No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC40.16306563391765186No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG40.16306563391765186No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG40.16306563391765186No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT40.16306563391765186No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT40.16306563391765186No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG40.16306563391765186No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG40.16306563391765186No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA40.16306563391765186No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT40.16306563391765186No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA40.16306563391765186No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT40.16306563391765186No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.16306563391765186No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG40.16306563391765186No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATC40.16306563391765186No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT30.12229922543823889No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC30.12229922543823889No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG30.12229922543823889No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT30.12229922543823889No Hit
GGGCAAGAATCAAAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGT30.12229922543823889No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT30.12229922543823889No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT30.12229922543823889No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT30.12229922543823889No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT30.12229922543823889No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA30.12229922543823889No Hit
ATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTC30.12229922543823889No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC30.12229922543823889No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC30.12229922543823889No Hit
ATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG30.12229922543823889No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.12229922543823889No Hit
GTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCA30.12229922543823889No Hit
GTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGAT30.12229922543823889No Hit
ACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAG30.12229922543823889No Hit
CTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCC30.12229922543823889No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA30.12229922543823889No Hit
CACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGA30.12229922543823889No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC30.12229922543823889No Hit
TTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.12229922543823889No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT30.12229922543823889No Hit
GGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCA30.12229922543823889No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC30.12229922543823889No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG30.12229922543823889No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA30.12229922543823889No Hit
TACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGAT30.12229922543823889No Hit
ATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACA30.12229922543823889No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG30.12229922543823889No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT30.12229922543823889No Hit
ATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACATATAGGAACATG30.12229922543823889No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCC30.12229922543823889No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT30.12229922543823889No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA30.12229922543823889No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG30.12229922543823889No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG30.12229922543823889No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC30.12229922543823889No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG30.12229922543823889No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC30.12229922543823889No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG30.12229922543823889No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAA30.12229922543823889No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT30.12229922543823889No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT30.12229922543823889No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG30.12229922543823889No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT30.12229922543823889No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT30.12229922543823889No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC30.12229922543823889No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG30.12229922543823889No Hit
ATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCAT30.12229922543823889No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.12229922543823889No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC30.12229922543823889No Hit
GTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAA30.12229922543823889No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA30.12229922543823889No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA30.12229922543823889No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.12229922543823889No Hit
GTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAG30.12229922543823889No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT30.12229922543823889No Hit
ACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC30.12229922543823889No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG30.12229922543823889No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG30.12229922543823889No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT30.12229922543823889No Hit
CTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGTGCATGGGGTTCAGT30.12229922543823889No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA30.12229922543823889No Hit
GCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAACCGGTTCATTG30.12229922543823889No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA30.12229922543823889No Hit
GTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTG30.12229922543823889No Hit
GTTCATTGATGCTTAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATC30.12229922543823889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGTG100.0066678347145.02
Produced by FastQC (version 0.11.5)