FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAGCTCTCTTCAGTTCCTTCTAAAAACCTCAACTTACGGAAGATGGG13.7037037037037033No Hit
GTACAAAGAGAACCGGTTAATTGAAATTGGAGTAACACCGAGGGAAGTCC13.7037037037037033No Hit
GTCTGGGCTCCCAGTATGGGGCAATGAAAAGAAGGACAAACTGGCAAATG13.7037037037037033No Hit
TCCAAACACAGTTAGCCATAACAGTACCCTGTTATAATGAAATAAGCCCA13.7037037037037033No Hit
AGGTAAAGAGTTCACCCACCTGGAAAAAAGAATAGAGAATTTAAATAAAA13.7037037037037033No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG13.7037037037037033No Hit
TTATGCAAAAAACTCAAGAGAATGTCAAAGGCGTAAAGTCGCAGCATTGC13.7037037037037033No Hit
GCATGAGGACATGCTGCCGTTACACCTTTGTTCGAGTCATGATTGGGCCA13.7037037037037033No Hit
CTGCAGAGGAGATGCCCACCAAAGCGGACTACACCCTTGACGAAGAGAGC13.7037037037037033No Hit
GTGTCATCTATTAGAAGAGGCCTTATTTTCTCAAGTTTCTTCTTTGTTGA13.7037037037037033No Hit
GCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACATGCTA13.7037037037037033No Hit
GAATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCGTT13.7037037037037033No Hit
AATCGAAACTAACAAGTTTGCTGCAATATACACATTTGGAAGTTTGTTTC13.7037037037037033No Hit
ACGATTGACAGTAAGACAGAGATGACCGAGGCGCGACATGTGTTGCAACG13.7037037037037033No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC13.7037037037037033No Hit
TCAAGTAAGAGAAAGTCGAAACCCAGGAAACGCTGAGATTGAAGACCTCA13.7037037037037033No Hit
ACATATAGGTGCCATAGGGGCGACACACAAATTCAGACGAGAAGATCATT13.7037037037037033No Hit
ACCTTACACCACCAATCTGTCAGCTCTGGCACATTACAAAGAGAGATGCT13.7037037037037033No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC13.7037037037037033No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCA13.7037037037037033No Hit
AAACAAGGTCGTTTTTAAACAATTAGAACATAATTGAATGCCATCCGAAT13.7037037037037033No Hit
ACCCAGTGTGACTATCAGATACGCCATTTTAACCATTTGTTTTAAAAGAA13.7037037037037033No Hit
GGTACTTTATTGTACAGTATGAATAGCAAATAGTAGCATTGCCACAACTT13.7037037037037033No Hit
GGTATATAAAACTTATTTCGATAAGGTCGAAAGGTTGAAACATGGTACCT13.7037037037037033No Hit
CTATGGCACCTATATGTGTATCTGTAGACTTTGATGAACAATTGAAGAGC13.7037037037037033No Hit
AACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATG13.7037037037037033No Hit
ATCCCACCCAGGAATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTG13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)