FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA32.8301886792452833No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.9433962264150944No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA10.9433962264150944No Hit
GTCTTGTGTGTTGGCCCAAGTAAACTCTTGGGAGGGTTGTTAATTGCAGG10.9433962264150944No Hit
GTCCTGCACAGTAAATGAAACACAACAGGGGAAGAGAAACCTAAATTTAT10.9433962264150944No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT10.9433962264150944No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.9433962264150944No Hit
CGTTTAAGCTCTGCTTGGGAAAAATTTATAATATACATATGAGCCCTTGA10.9433962264150944No Hit
CTCTCCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC10.9433962264150944No Hit
CCCGTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACC10.9433962264150944No Hit
CAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCA10.9433962264150944No Hit
CCTTCCTTTTATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTT10.9433962264150944No Hit
GGTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATA10.9433962264150944No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGACCT10.9433962264150944No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG10.9433962264150944No Hit
CACTAGGACCACTGTGGACCAAATGGCCAAAAAACAACAGCAGACAAGAG10.9433962264150944No Hit
ATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTT10.9433962264150944No Hit
GATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGT10.9433962264150944No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG10.9433962264150944No Hit
GTCATGCTGTTTAGGGTCTCCAGCATCTTTGCGGTGGTGTAAGTCTACTT10.9433962264150944No Hit
AAGTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCT10.9433962264150944No Hit
CTTCAAAGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG10.9433962264150944No Hit
GTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGA10.9433962264150944No Hit
AACTTTGACCGCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGCG10.9433962264150944No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG10.9433962264150944No Hit
AGCTAATGGATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCC10.9433962264150944No Hit
CTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAGG10.9433962264150944No Hit
GGCCACTGATGTCTACTTCCAGTCTGCAGGTGCCCAAGGTGAAAGGGCTG10.9433962264150944No Hit
GCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGTCACTAAGCT10.9433962264150944No Hit
GTAGTGGCCCCCACTGTACATCCCAGCAGTCTGTAAGAATTCCAGTTTTC10.9433962264150944No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10.9433962264150944No Hit
TCTTTCTCCAGACAACCTATGACTAATTGAAAATTGCATCAGATGCCAAA10.9433962264150944No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT10.9433962264150944No Hit
CTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCC10.9433962264150944No Hit
CAACACTACGTCCCCTTGCATTAGCCTTCTCTCCTTTTGCAAGATTGCTC10.9433962264150944No Hit
CACTTGAGGCCCACCCCCATTGCTGTGCCAGACGCAACTCTACTCGCCCA10.9433962264150944No Hit
ATGTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGATTCAAT10.9433962264150944No Hit
GGACCTTACTGGCAAAATCTGTATTCAACAGTCTATATGCGTCTCCACAA10.9433962264150944No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10.9433962264150944No Hit
GCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCA10.9433962264150944No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC10.9433962264150944No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA10.9433962264150944No Hit
CCTTTGACGCCGCCCGTGTTCAGTCTCCAGGGTGCTGTTTCCCAGATGTA10.9433962264150944No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA10.9433962264150944No Hit
GTATGAGGCCTGTCCATTACTTGGTCCATCGGTCATTACAGTAAAGCAAG10.9433962264150944No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA10.9433962264150944No Hit
TTTTGGGTGCGGGGGTCTGGGTGTTTCTGGGCCGCTCGGGTTGGTTTGTG10.9433962264150944No Hit
CATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCTTTAGCTGTTA10.9433962264150944No Hit
AACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTG10.9433962264150944No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA10.9433962264150944No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC10.9433962264150944No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC10.9433962264150944No Hit
CTCTGGCCCTTTGCAGTGTTTGGACTTGAATTTGTGGGACATTAGGTCAA10.9433962264150944No Hit
GAAGAGGCACCACCCTTGGCCTCGATATCGACACAGCCACTCTTGTTGGG10.9433962264150944No Hit
CCTTTGACGCCCCACTTGTTCAGACTCCAGGGTGCTGTTTCCCAGATGTA10.9433962264150944No Hit
TTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACGAAACCTCTA10.9433962264150944No Hit
AGCGAAAGCAGGCCAGAGAGCTGATCAAAGTGCTGGAAGAGGCTAATCAG10.9433962264150944No Hit
GGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAAAGAA10.9433962264150944No Hit
ACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAGACT10.9433962264150944No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG10.9433962264150944No Hit
GTCGGGCTGTCAGTAAGTATGCTTGAGGCCCGTTTTGGTGTCATTACCAA10.9433962264150944No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA10.9433962264150944No Hit
GGTAAGTTATGTGGACTTCCATCCGTGTTACTCCAATTTGAATGATCCGG10.9433962264150944No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC10.9433962264150944No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC10.9433962264150944No Hit
TCCCAGCACTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAA10.9433962264150944No Hit
ACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCC10.9433962264150944No Hit
CTACAAAGAGGAGCTGTTTTTGCGGACAACTGCATTTTAACGGCATTCAG10.9433962264150944No Hit
CACTGTAAGCAGCGCGCGCCAGTACTGAGAAATGGGGAAGTGGGCGGCAG10.9433962264150944No Hit
TCTCAAAACCGTTTCTTGAACAAATGATTATAATTATCACTATCCAAACT10.9433962264150944No Hit
ACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGG10.9433962264150944No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10.9433962264150944No Hit
GTGCCTTCATCTGGATCTTCAGTCAATGCACCTGAATCCTTTCCAAGAAC10.9433962264150944No Hit
GATGGACACAAAAGCTGGCTACACCCTTGCAGAAGAGATCAGGTCAAGAA10.9433962264150944No Hit
CACTAAGACCACTGTGTACCATATGGCCATAATCAAAAAGTACACATCAG10.9433962264150944No Hit
GAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATATTGTCTTG10.9433962264150944No Hit
TTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGA10.9433962264150944No Hit
CCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACT10.9433962264150944No Hit
TGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGCATGGAAA10.9433962264150944No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTGTTCG10.9433962264150944No Hit
GATGGACCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGA10.9433962264150944No Hit
GATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTG10.9433962264150944No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA10.9433962264150944No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC10.9433962264150944No Hit
ACATAATGGCCTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT10.9433962264150944No Hit
GTCGAGGGGGCGGGGTGGCGTGGACAGGGGGCGGGGGCGGGGGAGGGGGC10.9433962264150944No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA10.9433962264150944No Hit
GGGCCATTGATCTCCCACATCATTGATGACGAATAAGTTATTGTCAACTT10.9433962264150944No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA10.9433962264150944No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC10.9433962264150944No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10.9433962264150944No Hit
TTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT10.9433962264150944No Hit
ATGTGCGGCTGGTCGGCTGGTGGGGGTGTCGGCTGGGTCGAGAGGGTTGG10.9433962264150944No Hit
GTGCGGCAATGCTTCGGTCCAATGATCGTCGAGCTTGCGGAAAAGGCTAT10.9433962264150944No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA10.9433962264150944No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10.9433962264150944No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC10.9433962264150944No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.9433962264150944No Hit
CAGGGTTCACAGCGGGATCAGGGGAGCTCTTTGACGGCAGCAGCTGGGAG10.9433962264150944No Hit
GGAAGGCGCTGTTGGGGCCATGTCTTCATAGGACGAGTGAAAGGATGCTA10.9433962264150944No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG10.9433962264150944No Hit
CTCTTCATTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCA10.9433962264150944No Hit
TGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGAGTCTCGATACTG10.9433962264150944No Hit
TCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGCTCGCGCAGAGAC10.9433962264150944No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers